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MAB5356 Anti-Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4

MAB5356
100 µg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtICC, IHC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5356
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4
References
Product Information
FormatPurified
Control
  • Eye
PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationAnti-Rhodopsin Antibody, C-terminus, last 9 amino acids, clone Rho 1D4 detects level of Rhodopsin & has been published & validated for use in IC, IH, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern blot: 1:100-1:500 using ECL. Suggested dilution buffer is PBS and blocking buffer is PBS containing 5% milk. Recognizes 36 kDa monomers, dimmers and trimers depending on sample preparation. Boiling induces the aggregation of rhodopsin.

Western blot of isolated bovine rod outer segment labeled for rhodopsin (~85% of rod outer segment protein) with the rho 1D4 antibody. Amount of rod outer segments applied to the SDS gel: (a) 2.5 mg; (b) 0.63 mg; (c) 0.16 mg; (d) 0.04 mg. At higher protein quantities, dimers, trimers and tetramers of rhodopsin can be observed along with the monomer.

Immunohisto/cytochemistry: 1:100-1:500 on paraformaldehyde and glutaraldehyde fixed frozen tissue sections. Preferred fixation is paraformaldehyde for 4 hours at 2-8°C. Suggested permeabilization method is 0.2% Triton X-100.

Immunoprecipitation

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenBovine rhodopsin
EpitopeC-terminus, last 9 amino acids
CloneRho 1D4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes Rhodopsin. Reacts with both native and recombinant protein. No known reactivity to other proteins.
IsotypeIgG1
Species Reactivity
  • Vertebrates
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryRetinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness.
Gene Symbol
  • RHO
  • rhodopsin
  • Opsin-2
  • OPN2
  • Rhodopsin
  • RP4
  • MGC138311
  • MGC138309
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08100 # Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
SIZE: 348 amino acids; 38893 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Rod shaped photoreceptor cells which mediates vision in dim light.
PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
DISEASE: SwissProt: P08100 # Defects in RHO are the cause of retinitis pigmentosa type 4 (RP4) [MIM:180380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP4 inheritance is autosomal dominant. & Defects in RHO are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. & Defects in RHO are a cause of autosomal dominant congenital stationary night blindness 1 (CSNBAD1) [MIM:610445]; also known as rhodopsin-related congenital stationary night blindness. Congenital stationary night blindness is a nonprogressive retinal disorder characterized by impaired night vision and ocular symptoms such as myopia, hyperopia, nystagmus and reduced visual acuity.
SIMILARITY: SwissProt: P08100 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Molecular Weight48 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MAB5356 04053252661259

Documentation

Anti-Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4 MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4 Certificati d'Analisi

TitoloNumero di lotto
MOUSE ANTI-RHODOPSIN MONOCLONAL ANTIBODY - 2028473 2028473
MOUSE ANTI-RHODOPSIN MONOCLONAL ANTIBODY - 2140032 2140032
MOUSE ANTI-RHODOPSIN MONOCLONAL ANTIBODY - 2118023 2118023
MOUSE ANTI-RHODOPSIN MONOCLONAL ANTIBODY - 2446771 2446771
MOUSE ANTI-RHODOPSIN - 3174791 3174791
MOUSE ANTI-RHODOPSIN - 3323041 3323041
MOUSE ANTI-RHODOPSIN - 3474164 3474164
MOUSE ANTI-RHODOPSIN - 3698126 3698126
MOUSE ANTI-RHODOPSIN - 3815810 3815810
MOUSE ANTI-RHODOPSIN - 3858180 3858180

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiApplicazioneSpecieCodice d'identificazione nel Pub Med
Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration.
Zhao, L; Zabel, MK; Wang, X; Ma, W; Shah, P; Fariss, RN; Qian, H; Parkhurst, CN; Gan, WB; Wong, WT
EMBO molecular medicine  7  1179-97  2015

Mostra il sommario
26139610 26139610
1D4: a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins.
Molday, LL; Molday, RS
Methods in molecular biology (Clifton, N.J.)  1177  1-15  2014

Mostra il sommario
24943310 24943310
Involvement of NT3 and P75(NTR) in photoreceptor degeneration following selective Müller cell ablation.
Shen, W; Zhu, L; Lee, SR; Chung, SH; Gillies, MC
Journal of neuroinflammation  10  137  2013

Mostra il sommario
24224958 24224958
Interaction of complement factor h and fibulin3 in age-related macular degeneration.
Wyatt, MK; Tsai, JY; Mishra, S; Campos, M; Jaworski, C; Fariss, RN; Bernstein, SL; Wistow, G
PloS one  8  e68088  2013

Mostra il sommario
23840815 23840815
Meckelin 3 is necessary for photoreceptor outer segment development in rat Meckel syndrome.
Tiwari, S; Hudson, S; Gattone, VH; Miller, C; Chernoff, EA; Belecky-Adams, TL
PloS one  8  e59306  2013

Mostra il sommario
23516626 23516626
Changes in retinal morphology, electroretinogram and visual behavior after transient global ischemia in adult rats.
Zhao, Y; Yu, B; Xiang, YH; Han, XJ; Xu, Y; So, KF; Xu, AD; Ruan, YW
PloS one  8  e65555  2013

Mostra il sommario
23776500 23776500
A novel experimental mouse model of retinal detachment: complete functional and histologic recovery of the retina.
Zeng, R; Zhang, Y; Shi, F; Kong, F
Investigative ophthalmology & visual science  53  1685-95  2011

Mostra il sommario
22323470 22323470
Functional and anatomical remodeling in human retinal detachment.
Clairton F de Souza,Michael Kalloniatis,Philip J Polkinghorne,Charles N J McGhee,Monica L Acosta
Experimental eye research  97  2011

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22406310 22406310
The 1D4 antibody labels outer segments of long double cone but not rod photoreceptors in zebrafish.
Yin, J; Brocher, J; Linder, B; Hirmer, A; Sundaramurthi, H; Fischer, U; Winkler, C
Investigative ophthalmology & visual science  53  4943-51  2011

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Zebrafish22743318 22743318
Selective loss of RPGRIP1-dependent ciliary targeting of NPHP4, RPGR and SDCCAG8 underlies the degeneration of photoreceptor neurons.
Patil, H, et al.
Cell Death Dis, 3: e355 (2012)  2011

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22825473 22825473