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04-1572-I Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

04-1572-I
100 µL  
Purchase on Sigma-Aldrich

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Panoramica

Replacement Information

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
MChIP, WB, ELISARCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-1572-I
Replaces04-1572
DescriptionAnti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
Alternate Names
  • DNA-directed RNA polymerase II subunit RPB1
  • RNA polymerase II subunit B1
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase III largest subunit
  • RNA-directed RNA polymerase II subunit RPB1
Background InformationRPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at 'Ser-7' of the heptapepdtide repeat. This antibody recognizes the 'Ser-5' CTD residue of RPB1.
References
Product Information
FormatCulture Supernatant
Control
  • Untreated and lambda phosphotase treated NIH/3T3 cell lysates
PresentationRat monoclonal IgG2aκ with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 is a highly specific rat monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting & ELISA.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Western Blotting
  • ELISA
Application NotesChromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).

ELISA Analysis: A representative lot was used by an independent laboratory to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminal domain (CTD).
EpitopePhosphorylated Ser5 at the C-terminus domain (CTD).
Clone3E8
HostRat
SpecificityThis antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminus domain (CTD).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human based upon immunogen design.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLR2A
  • POLR2
Purification MethodUnpurified
UniProt Number
Molecular Weight~220 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in untreated and lambda phosphotase treated NIH/3T3 cell lysates.

Western Blot Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-5) in 10 µg of untreated and lambda phosphotase treated NIH/3T3 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
04-1572-I 04053252880230

Documentation

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 Certificati d'Analisi

TitoloNumero di lotto
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 - 2395817 2395817
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 - 3733821 3733821
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 - 3914333 3914333
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 - 3934811 3934811
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 - 4097777 4097777
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 - Q2159799 Q2159799
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 -2676687 2676687
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 -2716779 2716779
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 -2720133 2720133
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5), clone 3E8 -2766522 2766522

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
In vivo live imaging of RNA polymerase II transcription factories in primary cells.
Ghamari, Alireza, et al.
Genes Dev., 27: 767-777 (2013)  2013

Mostra il sommario
23592796 23592796
Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7.
Chapman, Rob D, et al.
Science, 318: 1780-2 (2007)  2007

Mostra il sommario
18079404 18079404

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Categorie

Life Science Research > Antibodies and Assays > Primary Antibodies