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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

04-1569 Sigma-AldrichAnti-RNA polymerase II subunit B1 Antibody, clone 4F8

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II A & has been validated in WB, ELISA.

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

MSDS
Cert. d'Analisi
Riferimenti bibliografici

Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors, RNA Metabolism & Binding Proteins Gene Symbol: POLR2A, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 UniProt Number: P24928

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Tabella delle specifiche principali

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H	WB, ELISA, ChIP	R	Purified	Monoclonal Antibody

Description
Catalogue Number	04-1569
Description	Anti-RNA polymerase II subunit B1 Antibody, clone 4F8
Alternate Names	DNA-directed RNA polymerase II A DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase III largest subunit RNA polymerase II subunit B1 RNA-directed RNA polymerase II subunit RPB1 polymerase (RNA) II (DNA directed) polypeptide A (220kD) polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Background Information	RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.

References

Product Information
Format	Purified
Control	NIH/3T3 cell lysates
Presentation	Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II A & has been validated in WB, ELISA.
Key Applications	Western Blotting ELISA Chromatin Immunoprecipitation (ChIP)
Application Notes	Chromatin Immunoprecipitation Analysis: A representative lot was used by independent laboraotry in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)

Biological Information
Immunogen	Ovalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1.
Epitope	Unknown
Clone	4F8
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rat
Specificity	This antibody recognizes RNA polymerase II subunit B1.
Isotype	IgG1κ
Species Reactivity	Mouse Human
Species Reactivity Note	Demonstrated to react with mouse. Predicted to react with human based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000928
Entrez Gene Summary	This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq].
Gene Symbol	POLR2A POLR2 POLRA RPB1 RPBh1 RPO2 RPOL2 RpIILS hRPB220 hsRPB1
Purification Method	Protein G Purified
UniProt Number	P24928
UniProt Summary	FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. CATA;YTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). SUBUNIT STRUCTURE: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C By similarity. Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2. SUBCELLULAR LOCATION: Nucleus PTM: The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapepdtide repeat. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphataes, and a "CTD code" that specifies the position of Pol II within the transcription cycle has been proposed. MISCELLANEOUS: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucelotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion. SEQUENCE SIMILARITIES: Belongs to the RNA polymerase beta' chain family.
Molecular Weight	~ 220 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in NIH/3T3 cell lysates. Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II subunit B1 on 10 µg of NIH/3T3 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Numero di catalogo	GTIN
04-1569	04053252552182

Documentation

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 MSDS

Titolo
Scheda di sicurezza (MSDS)

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 Certificati d'Analisi

Titolo	Numero di lotto
Anti-RNA polymerase II subunit B1, clone 4F8	2470766
Anti-RNA polymerase II subunit B1, clone 4F8 - 2446745	2446745
Anti-RNA polymerase II subunit B1, clone 4F8 - 2064323	2064323
Anti-RNA polymerase II subunit B1, clone 4F8 - 2293534	2293534
Anti-RNA polymerase II subunit B1, clone 4F8 - 3156839	3156839
Anti-RNA polymerase II subunit B1, clone 4F8 - 3557814	3557814
Anti-RNA polymerase II subunit B1, clone 4F8 - 3856224	3856224
Anti-RNA polymerase II subunit B1, clone 4F8 - 4086892	4086892
Anti-RNA polymerase II subunit B1, clone 4F8 - NG1873218	NG1873218
Anti-RNA polymerase II subunit B1, clone 4F8 - NG1946568	NG1946568

Riferimenti bibliografici

Panoramica dei riferimenti bibliografici	Codice d'identificazione nel Pub Med
Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7. Chapman, Rob D, et al. Science, 318: 1780-2 (2007) 2007 Mostra il sommario RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Differential phosphorylation of serine-2 and serine-5 at the 5' and 3' regions of genes appears to coordinate the localization of transcription and RNA processing factors to the elongating polymerase complex. Using monoclonal antibodies, we reveal serine-7 phosphorylation on transcribed genes. This position does not appear to be phosphorylated in CTDs of less than 20 consensus repeats. The position of repeats where serine-7 is substituted influenced the appearance of distinct phosphorylated forms, suggesting functional differences between CTD regions. Our results indicate that restriction of serine-7 epitopes to the Linker-proximal region limits CTD phosphorylation patterns and is a requirement for optimal gene expression.	18079404

Panoramica dei riferimenti bibliografici

Codice d'identificazione nel Pub Med

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Categorie

Life Science Research > Antibodies and Assays > Primary Antibodies

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