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MABC112 Anti-RASSF1 Antibody, clone 5C9.1

Detect RASSF1 using this Anti-RASSF1 Antibody, clone 5C9.1 validated for use in western blotting & IHC.

Anti-RASSF1 Antibody, clone 5C9.1: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: RASSF1, RDA32 UniProt Number: Q9NS23
MABC112
100 µg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC112
ReplacesMABE526
DescriptionAnti-RASSF1 Antibody, clone 5C9.1
Alternate Names
  • Ras association domain-containing protein 1
Background InformationRASSF1, also known as Ras association domain-containing protein 1, and encoded by the gene RASSF1/RDA32, is an important cell cycle and cell proliferation regulator protein. RASSF1 acts as a tumor suppressor and operates within the death receptor dependent apoptotic pathways. Additionally, RASSF1 inhibits proliferation at the G1/S boundary by regulating the accumulation of cyclin D1 protein. RASSF1 is localized to the cytoplasm and cytoskeleton depending upon cellular environment. RASSF1 localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. The protein also co-localizes with MDM2 in the nucleus. RASSF1 is expressed in multiple isoforms but in generally it is ubiquitously expressed but down regulated in cancer and tumor cells.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect RASSF1 using this Anti-RASSF1 Antibody, clone 5C9.1 validated for use in western blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:50-500 dilution from a representative lot detected RASSF1 in human kidney and human prostate cancer tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human RASSF1.
Clone5C9.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RASSF1
  • RDA32
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~39 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected RASSF1 in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABC112 04053252943195