Millipore Sigma Vibrant Logo
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

MABC553 Anti-Pim-1 Antibody, C-terminus, clone 19F7

Anti-Pim-1 Antibody, C-terminus, clone19F7 is an antibody against Pim-1 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation and Immunocytochemistry.

Anti-Pim-1 Antibody, C-terminus, clone 19F7: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: PIM1 UniProt Number: P11309
MABC553
100 µg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

100% Performance Guaranteed

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P), IP, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC553
DescriptionAnti-Pim-1 Antibody, C-terminus, clone 19F7
Alternate Names
  • Serine/threonine-protein kinase pim-1
Background InformationPim-1 is a serine/threonine protein kinase encoded by the PIM1 (or PIM) gene in human. Pim-1 is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-Pim-1 Antibody, C-terminus, clone19F7 is an antibody against Pim-1 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Pim-1 in human liver and human prostate cancer section tissue.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Pim-1 in Transfected Hek293 (Cen, B., et al. (2010). Journal of Biol. Chem. 285(38): 29128-29137).
Immunocytochemistry Analysis: A representative lot detected Pim-1 in UT-SCC-12A UT-SCC-22 and UT-SCC-43A cells treated with EGF (Peltola, K., et al. (2009). NEO Plasia. 11(7):629-636).
Immunohistochemistry Analysis: A representative lot detected Pim-1 in adipocytic tumors (Nga, M.E., et al. (2010). Int. J. Exp. Path. 91:34-43).
Western Blotting Analysis: A representative lot detected Pim-1 in SCC-12 cells treated with Pim-1 siRNA (Peltola, K., et al. (2009). NEO Plasia. 11(7):629-636).
Western Blotting Analysis: A representative lot detected Pim-1 in transfected Hek293 cells (Cen, B., et al. (2010). Journal of Biol. Chem. 285(38): 29128-29137).
Biological Information
ImmunogenConjugated linear peptide corresponding to the C-terminus of human Pim-1.
EpitopeC-terminus
Clone19F7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PIM1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~38/41 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Pim-1 in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABC553 04055977159288