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MABS522 Anti-PIKFYVE Antibody, clone R159.4.3C9

Anti-PIKFYVE Antibody, clone R159.4.3C9 is an antibody against PIKFYVE for use in Western Blotting.

Anti-PIKFYVE Antibody, clone R159.4.3C9 : MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling Research Sub-Category: General Post-translation Modification Gene Symbol: PIKFYVE, CFD, FAB1, HEL37, KIAA0981, PIP5K, PIP5K3, ZFYVE29 UniProt Number: Q9Y2I7
MABS522
100 μg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS522
DescriptionAnti-PIKFYVE Antibody, clone R159.4.3C9
Alternate Names
  • 1-phosphatidylinositol 3-phosphate 5-kinase
  • Epididymis luminal protein 37
  • FYVE finger-containing phosphoinositide kinase
  • PIKfyve
  • Phosphatidylinositol 3-phosphate 5-kinase type III
  • PIPkin-III
  • Type III PIP kinase
  • Zinc finger FYVE domain containing 29
Background Information1-phosphatidylinositol 3-phosphate 5-kinase (EC 2.7.1.150; UniProt Q9Y2I7; also known as Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 29) is encoded by the PIKFYVE (also known as CFD, FAB1, HEL37, KIAA0981, PIP5K, PIP5K3, ZFYVE29) gene (Gene ID 200576) in human. PIKfyve is a class III lipid kinase that is responsible for phosphorylating phosphatidylinositol (PtdIns) and phosphatidylinositol 3-phosphate (PtdIns3P) of their inositol ring at the D-5 position to form PtdIns3P and PtdIns(3,5)P2 (phosphatidylinositol 3,5-bisphosphate), respectively. PIKfyve forms a stable ternary heterooligomeric complex with its regulator ArPIKfyve (encoded by the VAC14 gene) and the Sac1 domain-containing PtdIns(3,5)P2 5-phosphatase Sac3 (encoded by the FIG4 gene), and both ArPIKfyve and Sac3 are required for maximal PIKfyve activity. PIKfyve is also known to interact with the Rab9 effector RABEPK and the kinesin adaptor JLP (encoded by SPAG9). By regulating endosomal trafficking, PIKfyve plays an important role in multiple biological processes, such as GLUT4 translocation, retroviral budding, autophagy, and neurodegeneration. Human PIKfyve is initially produced as a 2098-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. PIKFYVE gene mutation is linked to corneal fleck dystrophy (CFD) characterized by white flecks scattered in all levels of the stroma.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PIKFYVE Antibody, clone R159.4.3C9 is an antibody against PIKFYVE for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected PIKFYVE in 10 µg of HepG2 cell lysate.
Biological Information
ImmunogenAviTag-6xHis-tagged recombinant protein corresponding an internal fragment of human PIKFYVE.
EpitopeInternal (N-terminal half).
CloneR159.4.3C9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with all four spliced isoforms of human PIKfyve reported by UniProt (Q9Y2I7-1 through Q9Y2I7-4).
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PIKFYVE
  • CFD
  • FAB1
  • HEL37
  • KIAA0981
  • PIP5K
  • PIP5K3
  • ZFYVE29
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~240/50 kDa observed. 237,1 kDa (isoform 1), 50.21 kDa (isoform 2), 51.48 kDa (isoform 3), 61.60 kDa (isoform 4) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PIKFYVE in 20 µg of HL-60 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABS522 04055977312478