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ABS1006 Anti-PCSK9 Antibody

ABS1006
100 µg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1006
DescriptionAnti-PCSK9 Antibody
Alternate Names
  • NARC-1
  • PC9
  • Subtilisin/kexin-like protease PC9
  • Proprotein convertase 9
  • Neural apoptosis-regulated convertase 1
  • Proprotein convertase subtilisin/kexin type 9
Background InformationPCSK9 protein is an important protein in the regulation of plasma cholesterol homeostasis. PCSK9 binds various lipid receptor protein members including low density lipoprotein receptors (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2) and promotes their degradation or sequestration. In neurons, PCSK9 regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. PCSK9 is localized in cytoplasm but it is also secreted and thus can be found in endosomes, lysosomes, and endoplasmic reticulum and Golgi as well. PCSK9 is expressed in normal cells such as Schwann cells and pancreatic epithelial cells but PCSK9 is also expressed in many cancers and cancer cells lines as well.
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 30% glycerol.
Quality LevelMQ100
Applications
ApplicationThis Anti-PCSK9 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of PCSK9.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot of this antibody detected PCSK9 protein in treated HepG2 cell lysate (Jeong, H. J., et al. J. Lipid Res. 2008. 49: 399–409.)
Western Blotting Analysis: A representative lot of this antibody detected PCSK9 protein in siRNA transfected HepG2 cells without and with BBR treatment (Data compliments of Sahng Wook Park of Yongsei University College of Medicine).
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to Human PCSK9.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PCSK9
  • NARC1
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~75 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry on HepG2 cells
Immunocytochemistry Analysis: 8.0 µg/mL of this antibody detected PCSK9 in HepG2 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µg
Global Trade Item Number
Numero di catalogo GTIN
ABS1006 04055977112153