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ABT331 Anti-Nephrin Antibody

Detect Nephrin using this rabbit polyclonal antibody, Anti-Nephrin Antibody validated for use in western blotting, IHC & Immunofluorescence.

Anti-Nephrin Antibody: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: NPHS1 UniProt Number: O60500
ABT331
100 µg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, IHC, IFRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT331
DescriptionAnti-Nephrin Antibody
Alternate Names
  • Nephrin
  • NPHN
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor
Background InformationNephrin (NPHS1, NPHN), also known as is Renal glomerulus-specific cell adhesion receptor, is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
References
Product Information
FormatAffinity Purified
Control
  • Mouse kidney tissue lysate
PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Nephrin using this rabbit polyclonal antibody, Anti-Nephrin Antibody validated for use in western blotting, IHC & Immunofluorescence.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: 1 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.

Immunofluorescence Analysis: 10 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Nephrin.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Mouse
Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human based on immunogen design.
Antibody TypePolyclonal Antibody
Gene Symbol
  • NPHS1
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~100 kDa observed. Uncharacterized bands may appear in some lysate(s). Despite its calculated molecular weight of 135 kDa, Nephrin often migrates at a lower than expected size in SDS-PAGE.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Nephrin in 15 µg of mouse kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ABT331 04053252923524