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MABT857 Anti-Muscle Satellite Cells Antibody, clone SM C-2.6

Anti-Muscle Satellite Cells, clone SM C/2.6, Cat. No. MABT857, is a rat monoclonal antibody that detects muscle satellite cells and has been tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Fluorescence Activated Cell Sorting (FACS).

Anti-Muscle Satellite Cells Antibody, clone SM C-2.6: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Cell Structure UniProt Number: NA
MABT857
100 μg  
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, IF, IHC, FACSRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT857
DescriptionAnti-Muscle Satellite Cells Antibody, clone SM C-2.6
Background InformationSkeletal muscle satellite cells are quiescent mononucleated myogenic cells that reside between the sarcolemma and basement membrane of terminally-differentiated muscle fibers. They serve as a reserve population of cells with stem-like properties. The number of satellite cells are shown to be highest in postnatal muscle, but declines with age. In their quiescent stage, satellite cells are rounded and mononucleated, and do not express relevant amounts of myogenic proteins. Also, in their quiescent state they are shown to be c-kit-, Sca-1-, CD34+, and CD45-. Satellite cells can proliferate in response to injury and regenerate muscle and produce more satellite cells. Hence, they have been considered to be the only myogenic source for the maintenance of skeletal muscle. When muscle regeneration is initiated, satellite cells changes from their quiescent state to an active stage and begin to proliferative. They can proliferate and then either fuse with each other to form myotubes, which mature into myofibers, or they can fuse with damaged segments of muscle fibers. (Ref.: Fukuda, S et al. (2004). Exp. Cell Res. 296(2); 245-255; Morgan JE and Partridge, TA (2003). Int. J. Biochem. Cell Biol. 35(8); 1151 1156).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Muscle Satellite Cells, clone SM C/2.6, Cat. No. MABT857, is a rat monoclonal antibody that detects muscle satellite cells and has been tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Fluorescence Activated Cell Sorting (FACS).
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry
  • Fluorescence Activated Cell Sorting (FACS)
Application NotesFluorescence Activated Cell Sorting (FACS) Analysis: A representative lot detected Muscle Satellite Cells in Fluorescence Activated Cell Sorting (FACS) applications (Urbani, L., et. al. (2012). PLoS One. 7(11):e49860).

Flow Cytometry Analysis: A representative lot detected Muscle Satellite Cells in Flow Cytometry applications (Ikemoto, M., et. al. (2007). Mol Ther. 15(12):2178-85; Fukada, S., et. al. (2004). Exp Cell Res. 296(2):245-55).

Immunohistochemistry Analysis: A representative lot detected Muscle Satellite Cells in Immunohistochemistry applications (Fukada, S., et. al. (2004). Exp Cell Res. 296(2):245-55; Yao, Y., et. al. (2016). Nat Commun. 7:11415).

Immunofluorescence Analysis: A representative lot detected Muscle Satellite Cells in Immunofluorescence applications (Fukada, S., et. al. (2004). Exp Cell Res. 296(2):245-55).
Biological Information
ImmunogenC2/4 myogenic cells.
CloneSM/C-2.6
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone SM C-2.6 detects quiescent satellite cells from adult mouse muscle.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in C2C12 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Muscle Satellite Cells in a population of one million C2C12 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABT857 04054839352645

Documentation

Anti-Muscle Satellite Cells Antibody, clone SM C-2.6 Certificati d'Analisi

TitoloNumero di lotto
Anti-Muscle Satellite Cells, clone SM C/2.6 - 3116274A 3116274A
Anti-Muscle Satellite Cells, clone SM C/2.6 - 3270701 3270701
Anti-Muscle Satellite Cells, clone SM C/2.6 - 3897354 3897354
Anti-Muscle Satellite Cells, clone SM C/2.6 - 4003923 4003923
Anti-Muscle Satellite Cells, clone SM C/2.6 Monoclonal Antibody Q2925831
Anti-Muscle Satellite Cells, clone SM C/2.6 Monoclonal Antibody 3116274