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ABS549 Anti-MuSK Antibody

ABS549
100 µL  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHC, IPRbSerumPolyclonal Antibody
Description
Catalogue NumberABS549
DescriptionAnti-MuSK Antibody
Alternate Names
  • Muscle, skeletal receptor tyrosine-protein kinase
  • Muscle-specific tyrosine-protein kinase receptor
  • MuSK
  • Muscle-specific kinase receptor
Background InformationMuSK, also known as Muscle, skeletal receptor tyrosine-protein kinase, Muscle-specific tyrosine-protein kinase receptor, or Muscle-specific kinase receptor, and encoded by the gene MUSK, a key muscle specific receptor tyrosine kinase that plays a central role in the formation and maintenance of the neuromuscular junction (NMJ). The NMJ is the special synapse between the motor neuron and the skeletal muscle. MuSK activation in the myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in the subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors in the postsynaptic membrane. MuSK also plays a role in the CNS by mediating cholinergic responses, synaptic plasticity and memory function. MuSK is a membrane bound protein localized to the postsynaptic side of the cell membrane at the neuromuscular junction in muscle cells.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MuSK antibody is an antibody against MuSK for use in western blotting, IHC & IP.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: A representative lot detected MuSK in C2C12 cell lysate (Simeone, L., et al. (2010). The Journal of Neuro. 30(19):6620-6634).
Immunohistochemistry Analysis: A representative lot detected MuSK in mouse soleus muscle tissue (Simeone, L., et al. (2010). The Journal of Neuro. 30(19):6620-6634).
Immunoprecipitation Analysis: A representative lot immunoprecipitated MuSK in C2C12 cell lysate (Simeone, L., et al. (2010). The Journal of Neuro. 30(19):6620-6634).
Biological Information
ImmunogenRecombinant protein corresponding to the extracellular domain of mouse MuSK.
EpitopeExtracellular domain
HostRabbit
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MUSK
Purification MethodUnpurified
UniProt Number
Molecular Weight~110 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MuSK in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ABS549 04053252973864

Documentation

Anti-MuSK Antibody MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-MuSK Antibody Certificati d'Analisi

TitoloNumero di lotto
Anti-MuSK - Q2424381 Q2424381
Anti-MuSK - 3216005 3216005
Anti-MuSK - 3329599 3329599
Anti-MuSK - 3507304 3507304
Anti-MuSK - 3596848 3596848
Anti-MuSK - 4034306 4034306
Anti-MuSK - 4182476 4182476
Anti-MuSK -2673183 2673183
Anti-MuSK -2702142 2702142
Anti-MuSK -2819572 2819572

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
A mutation causes MuSK reduced sensitivity to agrin and congenital myasthenia.
Ben Ammar, Asma, et al.
PLoS ONE, 8: e53826 (2013)  2013

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23326516 23326516
Identification of Erbin interlinking MuSK and ErbB2 and its impact on acetylcholine receptor aggregation at the neuromuscular junction.
Simeone, Luca, et al.
J. Neurosci., 30: 6620-34 (2010)  2009

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20463225 20463225
Identification of developmentally regulated expression of MuSK in astrocytes of the rodent retina.
Cheusova, Tatiana, et al.
J. Neurochem., 99: 450-7 (2006)  2005

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16899069 16899069
Casein kinase 2-dependent serine phosphorylation of MuSK regulates acetylcholine receptor aggregation at the neuromuscular junction.
Cheusova, Tatiana, et al.
Genes Dev., 20: 1800-16 (2006)  2005

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16818610 16818610
Activation of muscle-specific receptor tyrosine kinase and binding to dystroglycan are regulated by alternative mRNA splicing of agrin.
Scotton, Patrick, et al.
J. Biol. Chem., 281: 36835-45 (2006)  2005

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17012237 17012237