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AB3712 Anti-Mannosidase II Antibody, For Golgi Apparatus identification

AB3712
100 µL  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MkICC, IHCRbSerumPolyclonal Antibody
Description
Catalogue NumberAB3712
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Mannosidase II Antibody, For Golgi Apparatus identification
Product Information
FormatSerum
PresentationRabbit serum. Liquid with 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect the Mannosidase II protein using this Anti-Mannosidase II, For Golgi Apparatus identification validated for use in ICC & IHC.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunocytochemistry on HeLa and COS7 cells

Immunohistochemistry on cold methanol fixed tissue.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified peptide from human mannosidase II.
EpitopeFor Golgi Apparatus identification
HostRabbit
SpecificityMannosidase II. This antibody is a useful marker for golgi apparatus identification.
Species Reactivity
  • Human
  • Monkey
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein which is a member of family 38 of the glycosyl hydrolases. The protein is located in the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus.
Gene Symbol
  • MAN2A1
  • GOLIM7
  • MANII
  • MANA2
  • EC 3.2.1.114
Non-Reactive Species
  • Mouse
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q16706 # Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
SIZE: 1144 amino acids; 131141 Da
SUBUNIT: Homodimer; disulfide-linked (By similarity).
SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II membrane protein (By similarity).
PTM: Glycosylated (By similarity).
SIMILARITY: SwissProt: Q16706 ## Belongs to the glycosyl hydrolase 38 family.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain unopened vial at -70C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°. For storage at -20C°, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µL
Global Trade Item Number
Numero di catalogo GTIN
AB3712 04053252728297