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ABS1013 Anti-MTHFR Antibody

Detect MTHFR using this Anti-MTHFR Antibody validated for use in Western Blotting.

Anti-MTHFR Antibody : MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: MTHFR UniProt Number: P42898
ABS1013
200 µL  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, PorcineWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABS1013
DescriptionAnti-MTHFR Antibody
Alternate Names
  • Methylenetetrahydrofolate reductase
  • MTHFR
Background InformationMethylenetetrahydrofolate reductase or 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20; UniProt P42898) is encoded by the MTHFR gene (Gene ID 4524) in human. MTHFR catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, the predominant circulatory form of folate and carbon donor for the re-methylation of homocysteine to methionine. Defects in MTHFR cause plasma homocysteine elevation or hperhomocysteinaemia, a risk factor for cerebrovascular, peripheral vascular, and coronary heart disease. Multiple transcription start sites and alternative splicing in the MTHFR gene result in two enzyme isoforms.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect MTHFR using this Anti-MTHFR Antibody validated for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected MTHFR in mice plasma, kidney and brain (Chew, T.W., et al. (2011). J. Nutr. 141(8):1475-1481.).
Western Blotting Analysis: A representative lot detected MTHFR in porcine liver, human lymphocytes, human fetal kidney, human adult kidney, cells transfected with MTHFR (Rozen, R. U.S. Patent 6,833,243 B2, December 21, 2004).
Western Blotting Analysis: A representative lot detected MTHFR in HepG2, mouse liver, and RAW264.7 (Mikael, L.G., and Rozen, R. (2008). Cardiovasc. Res. 80(1):151-158).
Western Blotting Analysis: A representative lot detected recombinant MTHFR protein, as well as endogenous MTHFR in human kidney (both fetal & adult), fetal human lung, and adult human aorta (Tran, P., et al. (2002). Mamm. Genome. 13(9):483-492).
Biological Information
ImmunogenRecombinant protein corresponding to human MTHFR.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityReacts with both isoforms (PMID 12370778).
Species Reactivity
  • Mouse
  • Human
  • Porcine
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MTHFR
Purification MethodUnpurified
UniProt Number
Molecular Weight~70/77 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MTHFR in 10 µg of RAW264.7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ABS1013 04055977173017