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MAB3369 Anti-MMP-3 Antibody, clone SL-1 IID4

MAB3369
100 µg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, IP, WB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3369
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MMP-3 Antibody, clone SL-1 IID4
Alternate Names
  • Stromelysin-1
  • Transin
  • Proteoglycanase
Background InformationMMPs are frequently expressed in malignant neoplastic cells and play crucial roles in tumor invasion and metastasis. Tissue inhibitors of matrix metalloproteinases (TIMPs) control the activitiy of MMPS. The tissue distribution of three major MMPs has been defined in human mammary pathology: 1) collagenase (MMP-1) which degrades fibrillar interstitial collagens; 2) a 72-k/Da gelatinase (MMP-2) which mainly degrades type IV collagen and denatured collagens; and 3) stromelysin (MMP-3) which has a wider range of action, degrading several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens.
Product Information
FormatPurified
Control
  • POSTIVE CONTROL:

    Human endometrium cells, placenta, bladder, breast, ovarian carcinomas.
PresentationPurified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MMP-3 Antibody, clone SL-1 IID4 is an antibody against MMP-3 for use in ELISA, IP, WB, IH(P).
Key Applications
  • ELISA
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunofluorescence

Immunoprecipitation (Use Protein A): 2μg/ml protein lysate.

Western blotting: 0.5-1.0 μg/ml for 2hrs at RT

Immunohistology (frozen & formalin-fixed): 10-20 μg/ml for 60 minute at room temperature. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissue sections.

Optimal working dilutions and protocols must be determined by end user.
Biological Information
ImmunogenAPMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1
CloneSL-1 IID4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityMAB3369 recognizes one doublet of 54kDa/59kDa and another doublet of 44kDa/49kDa, which are identified as unglycosylated and glycosylated species of pro (latent) and active forms of matrix metalloproteinase-3 (MMP-3; also known as Stromelysin-1 of Transin). MAB3369 shows no cross-reaction with the pro and active forms of other MMPs.

CELLULAR LOCALIZATION:

Cytoplasmic
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Gene Symbol
  • MMP3
  • STR1
  • Transin-1
  • STMY
  • MGC126102
  • STMY1
  • SL-1
  • MGC126103
  • MGC126104
  • transin-1
  • proteoglycanase
  • MMP-3
  • progelatinase
  • EC 3.4.24.17
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08254 # Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 477 amino acids; 53977 Da
DOMAIN: SwissProt: P08254 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsAntibody with sodium azide is stable for 12 months when stored at 2-8°C.
Packaging Information
Material Size100 µg
Global Trade Item Number
Numero di catalogo GTIN
MAB3369 04053252668715