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MABS1270 Anti-Lipoprotein Lipase/LPL Antibody, clone 4-1a

Anti-Lipoprotein Lipase/LPL Antibody, clone 4-1a is an antibody against Lipoprotein Lipase/LPL for use in Immunohistochemistry (Paraffin), Western Blotting, ELISA, Immunocytochemistry, Immunofluorescence.

Anti-Lipoprotein Lipase/LPL Antibody, clone 4-1a : MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling Research Sub-Category: Lipid Metabolism & Weight Regulation Gene Symbol: LPL, LIPD UniProt Number: P06858
MABS1270
100 μg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, BIH(P), WB, ELISA, ICC, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1270
DescriptionAnti-Lipoprotein Lipase/LPL Antibody, clone 4-1a
Alternate Names
  • Lipoprotein lipase
  • hLPL
  • LPL
Background InformationLipoprotein lipase (EC 3.1.1.34; UniProt P06858; also known as hLPL, LPL) is encoded by the LPL (also known as LIPD) gene (Gene ID 4023) in human. Lipoprotein lipase (LPL) catalyzes the hydrolysis of triglycerides in plasma lipoproteins. LPL is produced by adipocytes and myocytes and secreted into the interstitial spaces, where it is bound by GPIHBP1 (a glycosylphosphatidylinositol-anchored protein of capillary endothelial cells) and shuttled to the luminal face of capillaries. The GPIHBP1–LPL complex is crucial for the binding of triglyceride-rich lipoproteins (TRLs) to endothelial cells and the subsequent lipolytic processing of TRLs. TRLs bind only the LPL-GPIHBP1 complex, but not GPIHBP1 alone, on the cell surface. A deficiency of either protein results in severe hypertriglyceridemia (chylomicronemia) and impaired delivery of lipid nutrients to parenchymal cells. LPL is produced with a signal peptide sequence (a.a. 1-27), the removal of which yields the mature 448-amino acid (a.a. 28-475) enzyme containing a PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain (a.a. 341-464) and a heparin-binding domain (a.a. 346-441).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Lipoprotein Lipase/LPL Antibody, clone 4-1a is an antibody against Lipoprotein Lipase/LPL for use in Immunohistochemistry (Paraffin), Western Blotting, ELISA, Immunocytochemistry, Immunofluorescence.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
  • ELISA
  • Immunocytochemistry
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected lipoprotein lipase/LPL immunoreactivity in human pancreas, human skeletal muscle, and rat heart tissue sections.
Western Blotting Analysis: A representative lot detected human plasma lipoprotein lipase (LPL) and bovine milk LPL by Western blotting. Clone 4-1a exhibited much reduced binding to murine or chicken LPL, and failed to detect human hepatic lipase (hHL) (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Western Blotting Analysis: A representative lot detected recombinant wild-type human LPL (rhLPL), as well as rhLPL with mutated Gln3 & Arg4. Clone 4-1a failed to react with rhLPL with mutated Ile8 or Asp9, nor rhLPL with either a.a. 5-15 or aa.16-25 deletion (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
ELISA Analysis: A representative lot was employed as the capture antibody for the detection of human LPL by sandwich ELISA, using biotinylated clone 5D2 as the detcting antibody (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Immunocytochemistry Analysis: A representative lot detected the binding of human LPL to CHO and CHL cells transfected to express surface GPIHBP1, but not to mock transfected cells or cells transfected to express GPIHBP1 C65S mutant. Clone 4-1a does not compete against triglyceride-rich lipoproteins (TRLs) for binding the LPL-GPIHBP1 complex on cell surface (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Immunofluorescence Analysis: A representative lot detected hLPL immunoreactivity associated with GPIHBP1 on capillary endothelial cells in OCT-embedded, methanol/acetone-fixed frozen skeletal muscle sections from mice expressing muscle-specific human LPL transgene. Much lower immunoreactivity of the endogenous mLPL was detected in skeletal muscle sections from non-transgenic mice (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Note: Clone 4-1a exhibits much weaker affinity toward rat & murine LPL when compared with human LPL. Higher amount of sample loading (in Western blotting and ELISA) and/or high sensitivity detection method (e.g. HRP polymer or fluorescence detection in Western blotting and immunohistochemistry) is recommended when using this clone for detecting rat & murine LPL.
Biological Information
ImmunogenPurified human plasma lipoprotein lipase/LPL.
EpitopeNear the N-terminus of mature LPL.
Clone4-1a
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4-1a binds human and bovine LPL, as well as GPIHBP1-bound LPL, but not human hepatic lipase. Clone 4-1a does not inhibit LPL catalytic activity or LPL heparin interaction, nor does clone 4-1a compete against triglyceride-rich lipoproteins (LRLs) for bining GPIHBP1-LPL complex (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Bovine
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LPL
  • LIPD
Purification MethodProtein G Purified
UniProt Number
Molecular Weight53.16 kDa (ProLPL) and 50.39 (Mature LPL) calculated. ~56 kDa observed (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in mouse heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected lipoprotein lipase/LPL immunoreactivity in mouse heart tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABS1270 04055977333336

Documentation

Anti-Lipoprotein Lipase/LPL Antibody, clone 4-1a MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Lipoprotein Lipase/LPL Antibody, clone 4-1a Certificati d'Analisi

TitoloNumero di lotto
Anti-Lipoprotein Lipase/LPL, -Q2664685 Q2664685
Anti-Lipoprotein Lipase/LPL, clone 4-1a - 3393263 3393263
Anti-Lipoprotein Lipase/LPL, clone 4-1a - 3862595 3862595
Anti-Lipoprotein Lipase/LPL, clone 4-1a - 3899555 3899555
Anti-Lipoprotein Lipase/LPL, clone 4-1a - 3937289 3937289
Anti-Lipoprotein Lipase/LPL, clone 4-1a - 3948153 3948153
Anti-Lipoprotein Lipase/LPL, clone 4-1a - 3979948 3979948
Anti-Lipoprotein Lipase/LPL, clone 4-1a Monoclonal Antibody 3043529