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05-1066-25UG Anti-Insulin Antibody, clone E11D7 (mouse monoclonal)

Anti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P).

Anti-Insulin Antibody, clone E11D7 (mouse monoclonal): MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling, Metabolism Research Sub-Category: Insulin/Energy Signaling, Glucose/Glycogen Metabolism, Metabolic Hormones & Receptors Gene Symbol: INS, proinsulin, insulin UniProt Number: P01308
05-1066-25UG
25 μg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RELISA, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue Number05-1066-25UG
DescriptionAnti-Insulin Antibody, clone E11D7 (mouse monoclonal)
Background InformationInsulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary agent in carbohydrate homeostasis, it has effects on fat metabolism.
Product Information
FormatPurified
PresentationPurified mouse monolconal IgG1 in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05%NaN3
Quality LevelMQ100
Applications
ApplicationAnti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P).
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
Application NotesELISA (Direct) Analysis:
Insulin-coated plate was incubated with Anti-Insulin, clone E11D7 in 2-fold serial dilutions diluted in PBST from 5 μg/mL to 0.01 μg/mL.
Biological Information
ImmunogenHuman recombinant Insulin
EpitopeInsulin
CloneE11D7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityRecognizes insulin
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman tested. Not tested with other species, but expected to react with Mouse and Rat based on sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAfter removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Gene Symbol
  • INS
  • proinsulin
  • insulin
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Molecular Weight6 kDa
Product Usage Statements
Quality AssuranceImmunohistochemistry Analysis:
20 μg/mL of this antibody detected insulin in β cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size25 μg
Global Trade Item Number
Numero di catalogo GTIN
05-1066-25UG 04054839337710