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04-902 Anti-HA Tag Antibody, clone DW2, rabbit monoclonal

04-902
100 µL  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtWBRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-902
Replaces05-902
DescriptionAnti-HA Tag Antibody, clone DW2, rabbit monoclonal
Background InformationHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
References
Product Information
FormatCulture Supernatant
Control
  • HA-tagged WNT-1 protein.
PresentationCultured supernatant in 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.
Biological Information
ImmunogenKLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.

CloneDW2
HostRabbit
SpecificityRecognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.

IsotypeIgG
Species Reactivity
  • Vertebrates
Species Reactivity NoteMost common vertebrate species tested. Reactivity with other species has not been confirmed.
Antibody TypeMonoclonal Antibody
Molecular WeightVarious
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceWestern Blot Analysis
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.

Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
04-902 04053252738203

Documentation

Anti-HA Tag Antibody, clone DW2, rabbit monoclonal MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-HA Tag Antibody, clone DW2, rabbit monoclonal Certificati d'Analisi

TitoloNumero di lotto
Anti-HA Tag, clone DW2 - 2444134 2444134
Anti-HA Tag, clone DW2 - 2299155 2299155
Anti-HA Tag, clone DW2 - 2957992 2957992
Anti-HA Tag, clone DW2 - 3593758 3593758
Anti-HA Tag, clone DW2 - 3886618 3886618
Anti-HA Tag, clone DW2 - 4045806 4045806
Anti-HA Tag, clone DW2 - DAM1561709 DAM1561709
Anti-HA Tag, clone DW2 -2660175 2660175
Anti-HA Tag, clone DW2 -2683839 2683839
Anti-HA Tag, clone DW2 -2819147 2819147