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MAB2510 Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2

MAB2510
100 µg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
AIP, WB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2510
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2
Alternate Names
  • GFP
Background InformationThe Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Product Information
FormatPurified
PresentationPurified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2 is an antibody against Green Fluorescent Protein for use in IP, WB & IC.
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry

Immunoblotting: 1:1000

Immunoprecipitation

Optimal working dilutions and protocols must be determined by the end user.
Biological Information
Immunogenhighly-purified native GFP
Epitopeoriginal GFP only
Clone264-449-2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityDirected against a bacterially-expressed fusion protein. Also reacts with red-shifted GFP-variant. Does not react with enhanced GFP.
IsotypeIgG1
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
UniProt Number
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C for up to 12 months.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
Packaging Information
Material Size100 µg
Global Trade Item Number
Numero di catalogo GTIN
MAB2510 04053252396700