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AB1783 Anti-Glutamate Transporter Antibody, Glial

AB1783
50 µL  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RIH(P), IF, WBGpSerumPolyclonal Antibody
Description
Catalogue NumberAB1783
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Glutamate Transporter Antibody, Glial
Alternate Names
  • GLT-1
  • EAAT2
Background InformationGlutamate transporters (GluT) function to remove L-glutamate (Glu), the primary excitatory neurotransmitter in the mammalian central nervous system (CNS), from the synaptic cleft. By clearing the synapse in this manner Glu can be recycled for later use, the proper diffusion gradient can be maintained and excitotoxicity can be prevented. There have been reports of many different members of a GluT multigene family; GLT1, GLAST, EAAC1 and EAAT2. mRNA has been identified in brain for each of these proteins.

EAAT2 transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
References
Product Information
FormatSerum
HS Code3002 15 90
Control
  • Rat brain tissue
PresentationGuinea pig antiserum containing no preservatives.
Quality LevelMQ100
Applications
ApplicationDetect Glutamate Transporter using this Anti-Glutamate Transporter Antibody, Glial validated for use in IH(P), IF & WB.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
  • Western Blotting
Application NotesEvaluated by Western Blot on mouse brain membrane lysates.

Western Blot: 1:500 dilution of this antibody detected GLT-1 on 10 ug of mouse brain membrane lysates.

Immunohistochemistry:
1:1,000-1:4,000 dilution from a previuos lot used a DAB detection system on adult rat forebrain fixed with 4% paraformaldehyde.

Immunohistochemistry:
1:5,000-10,000 dilution from a previuos lot used a cyanine conjugated secondary antibody.

Enzymatic detection requires substantially higher primary antibody dilutions. Lightly fixed 4% PFA material is recommended.

Notes:

Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with 50 mL of Ca2+-free Tyrode+s solution followed by a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) for 6 minutes. Tissues were rapidly dissected out, postfixed in the same fixative for 90 minutes and rinsed for at least 24 hours in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Sections were cut (14 um) in a cryostat and incubated at 4°C overnight with AB1783 (1:5,000-1:10,000). After rinsing in PBS sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.



Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide from the carboxy-terminus of rat GLT-1.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostGuinea Pig
SpecificityGlial Glutamate Transporter GLT-1 (EAAT2). The antibody has been tested on central nervous system tissue. Preabsorption of the antiserum with the immunogen peptide (Catalog number AG391) completely abolishes the immunostaining.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Gene Symbol
  • SLC1A2
  • GLT-1
  • EAAT2
  • GLT1
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P43004 # Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
SIZE: 574 amino acids; 62104 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
PTM: Glycosylated.
SIMILARITY: SwissProt: P43004 ## Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family.
Molecular Weight~62 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot on mouse brain membrane lysates.

Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
AB1783 04053252509391

Documentation

Anti-Glutamate Transporter Antibody, Glial MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Glutamate Transporter Antibody, Glial Certificati d'Analisi

TitoloNumero di lotto
Anti-Glutamate Transporter, Glial - 2118014 2118014
Anti-Glutamate Transporter, Glial - 2384729 2384729
Anti-Glutamate Transporter, Glial - 2459592 2459592
Anti-Glutamate Transporter, Glial - 1960854 1960854
Anti-Glutamate Transporter, Glial - 2015978 2015978
Anti-Glutamate Transporter, Glial - 2112531 2112531
Anti-Glutamate Transporter, Glial - 2196029 2196029
Anti-Glutamate Transporter, Glial - 2234155 2234155
Anti-Glutamate Transporter, Glial - 2489069 2489069
Anti-Glutamate Transporter, Glial - 3305889 3305889

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiApplicazioneSpecieCodice d'identificazione nel Pub Med
Buyang Huanwu decoction increases the expression of glutamate transporter-1 and glutamate synthetase in association with PACAP-38 following focal ischemia.
Ding, W; Yu, P; Liu, W; Zhou, L; Guan, LI; Lin, R
Biomedical reports  3  651-656  2015

Mostra il sommario
26405540 26405540
Novel treatment with neuroprotective and antiviral properties against a neuroinvasive human respiratory virus.
Brison, E; Jacomy, H; Desforges, M; Talbot, PJ
Journal of virology  88  1548-63  2014

Mostra il sommario
24227863 24227863
Localization of excitatory amino acid transporters EAAT1 and EAAT2 in human postmortem cortex: a light and electron microscopic study.
Roberts, RC; Roche, JK; McCullumsmith, RE
Neuroscience  277  522-40  2014

Mostra il sommario
25064059 25064059
Astrocytosis in parkinsonism: considering tripartite striatal synapses in physiopathology?
Charron, G; Doudnikoff, E; Canron, MH; Li, Q; Véga, C; Marais, S; Baufreton, J; Vital, A; Oliet, SH; Bezard, E
Frontiers in aging neuroscience  6  258  2014

Mostra il sommario
Immunohistochemistry25309435 25309435
Activity of D-amino acid oxidase is widespread in the human central nervous system.
Sasabe, J; Suzuki, M; Imanishi, N; Aiso, S
Frontiers in synaptic neuroscience  6  14  2014

Mostra il sommario
24959138 24959138
Decreased EAAT2 protein expression in the essential tremor cerebellar cortex.
Lee, M; Cheng, MM; Lin, CY; Louis, ED; Faust, PL; Kuo, SH
Acta neuropathologica communications  2  157  2014

Mostra il sommario
Western Blotting, Immunohistochemistry25391854 25391854
Cxcl12/Cxcr4 signaling controls the migration and process orientation of A9-A10 dopaminergic neurons.
Yang, S; Edman, LC; Sánchez-Alcañiz, JA; Fritz, N; Bonilla, S; Hecht, J; Uhlén, P; Pleasure, SJ; Villaescusa, JC; Marín, O; Arenas, E
Development (Cambridge, England)  140  4554-64  2013

Mostra il sommario
Immunohistochemistry24154522 24154522
Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body - Implications for physiological functions.
Blosa, M, et al.
Neuroscience, 228: 215-34 (2013)  2013

Mostra il sommario
23069754 23069754
Lesion-induced alterations in astrocyte glutamate transporter expression and function in the hippocampus.
Schreiner, AE; Berlinger, E; Langer, J; Kafitz, KW; Rose, CR
ISRN neurology  2013  893605  2013

Mostra il sommario
24078881 24078881
Mislocalization of the exitatory amino-acid transporters (EAATs) in human astrocytoma and non-astrocytoma cancer cells: effect of the cell confluence.
Varini, K; Benzaria, A; Taïeb, N; Di Scala, C; Azmi, A; Graoudi, S; Maresca, M
Journal of biomedical science  19  10  2011

Mostra il sommario
22296701 22296701

Brochure

Titolo
Pathways and Biomarkers of Glutamatergic Synapse Flyer