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05-1540 Anti-Gemin2 Antibody, clone 2E17

Anti-Gemin2 Antibody, clone 2E17 is a Mouse Monoclonal Antibody for detection of Gemin2 also known as SMN interacting protein 1-delta & has been validated in WB, ICC, ELISA & IP.

Anti-Gemin2 Antibody, clone 2E17: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins, Neurodegenerative Diseases Gene Symbol: SIP1, SIP1-delta UniProt Number: O14893
05-1540
100 µg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, ELISA, IPMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1540
DescriptionAnti-Gemin2 Antibody, clone 2E17
Alternate Names
  • GEMIN2
  • Gemin2
  • Component of gems
  • SMN interacting protein 1-delta
  • SMN-interacting protein 1
  • survival interacting protein 1
  • survival of motor neuron protein interacting protein
Background InformationGemin-2 (also known as SIP1 or SMN interacting protein1) is a part of the SMN complex. The SMN complex plays a role in RNA metabolism and in splicing regulation. As part of the SMN complex Gemin-2 interacts directly with Gemin-5 in a heteromeric complex that includes Gemin-3, Gemin-4, Gemin-6. This macromolecular complex (SMN complex) mediates assembly of spliceosomal snRNA complexes containing U1 and U5.
References
Product Information
FormatPurified
Control
  • HeLa cell lysates.
PresentationPurified mouse IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-Gemin2 Antibody, clone 2E17 is a Mouse Monoclonal Antibody for detection of Gemin2 also known as SMN interacting protein 1-delta & has been validated in WB, ICC, ELISA & IP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • ELISA
  • Immunoprecipitation
Application NotesImmunocytochemistry:
Cited in Battle, D.J., et. al (2007). J. Biol. Chem. 282: 27953 - 27959 and Zhang, Z., et. al (2008). Cell, 133:85-600. using a representative lot.
ELISA:
Cited in Zhang, Z., et. al (2008). Cell, 133:85-600.
Immunoprecipitation:
Cited in Battle, D.J., et. al(2007). J. Biol. Chem. 282: 27953 - 27959 using a representative lot.
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to human Gemin-2.
EpitopeN/A
Clone2E17
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityAntibody recognizes Gemin-2.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteReacts with Human. Predicted to recognize mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SIP1
  • SIP1-delta
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFunction The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.

Subunit structure Part of the core SMN complex that contains SMN1, SIP1/GEMIN2, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with GEMIN5.
Molecular Weight32 kDa Calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Gemin-2 in HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
05-1540 04053252731624