Millipore Sigma Vibrant Logo
Attenzione: Ci siamo trasferiti. Non è più possibile acquistare i prodotti Merck Millipore nel sito MerckMillipore.comPer saperne di più

ABS1028 Anti-GPR56, C-terminal Antibody

ABS1028
100 µg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IP, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1028
DescriptionAnti-GPR56, C-terminal Antibody
Alternate Names
  • GPR56 seven-transmembrane subunit
  • GPR56 7TM
  • GPR56 subunit beta
  • GPR56 CT
  • GPR56©
  • TM7XN1
Background InformationG-protein coupled receptor 56 (GPR56) is a ubiquitous adhesion receptor, which belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is abundantly expressed in the in thyroid gland, brain, heart, and various tumor cells. Previous studies have suggested that GPR56 may inhibit the progression and metastasis of melanomas by interaction with extracellular-matrix proteins, such as Tissue Transglutaminase. GPR56 also plays a role in regulating VEGF production and angiogenesis via a PKCα-mediated pathway. GPR56 also plays a critical role in the development of the frontal cortex; previous studies have reported that mutations in GPR56 result in disorganized cortical lamination, which is most pronounced in the frontal cortex. This condition is known as bilateral frontoparietal polymicrogyria or BFPP.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-GPR56, C-terminal Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of GPR56, C-terminal.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesImmunoprecipitation Analysis: A representative lot of this antibody immunoprecipated GPR56 from rat kidney cell lysate and visualized through western blot (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Western Blot Analysis: A representative lot of this antibody detected GPR56 from rat kidney cell lysate (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Immunocytochemistry Analysis: A representative lot of this antibody detected GPR56 from transfected HEK293 cells (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Biological Information
Immunogenlinear peptide corresponding to the C-terminus of Mouse GPR56.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GPR56
  • TM7LN4
  • TM7XN1
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~ 120 kDa observed
This receptor is heavily glycosylated in cells, and thus the apparent molecular mass of the full-length receptor in most cell types is around 90-120 kDa. Moreover, this receptor is prone to extensive post-translational processing and proteolysis, and thus depending on the cell type the most prominent immunoreactive bands on Western blot are usually around 45 kDa, 25 kDa and/or 15 kDa in size
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Hela cell lysate.

Western Blotting Analysis: 1 µg/ml of this antibody detected GPR56 in 10 µg of Hela cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ABS1028 04053252993084

Documentation

Anti-GPR56, C-terminal Antibody MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-GPR56, C-terminal Antibody Certificati d'Analisi

TitoloNumero di lotto
Anti-GPR56, C-terminal - Q2452353 Q2452353
Anti-GPR56, C-terminal Polyclonal Antibody 3156025
Anti-GPR56, C-terminal Polyclonal Antibody 2920714
Anti-GPR56, C-terminal Polyclonal Antibody 3022939

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
The N terminus of the adhesion G protein-coupled receptor GPR56 controls receptor signaling activity.
Paavola, Kevin J, et al.
J. Biol. Chem., 286: 28914-21 (2011)  2010

Mostra il sommario
21708946 21708946