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ABN1466 Anti-GLS

Anti-GLS, Cat. No. ABN1466, is a highly specific rabbit polyclonal antibody that targets Glutaminase kidney isoform, mitochondrial, and has been tested in Western Blotting.

Anti-GLS: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Neuroscience Gene Symbol: GLS, GLS1, KIAA0838 UniProt Number: O94925
ABN1466
100 μg  
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1466
DescriptionAnti-GLS
Alternate Names
  • Glutaminase kidney isoform
  • mitochondrial
  • K-glutaminase
  • L-glutamine amidohydrolase
Background InformationGlutaminase kidney isoform, mitochondrial (UniProt: O94925; also known as EC:3.5.1.2, GLS, K-glutaminase, L-glutamine amidohydrolase) is encoded by the GLS (also known as GLS1, KIAA0838) gene (Gene ID: 2744) in human. GLS catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. It is primarily expressed in the brain and kidney and plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. During normal acid-base balance, the kidney extraction of plasma glutamine is low, however, during metabolic acidosis, about one third of the plasma glutamine is extracted and metabolized in a single pass through kidneys. Three isoforms of glutaminase have been described in humans. They are produced via alternate splicing. Isoform 1 and 3 are activated by phosphate, while the isoform 3 lacks catalytic activity. Isoform 1 is highly expressed in brain and kidney, but is not detected in liver. On the other hand, isoform 3 is highly expressed in astrocytoma, ganglioglioma, ependymoma. Lower levels of isoform 3 are reported in placenta, lung, pancreas and kidney, but it is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GLS, Cat. No. ABN1466, is a highly specific rabbit polyclonal antibody that targets Glutaminase kidney isoform, mitochondrial, and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected GLS in 10 µg of human kidney tissue lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects the Glutaminase kidney isoform, mitochondrial, in human. Also detects GLS in the neural cortex. It targets an epitope within 16 amino acids fromt he C-terminal region.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GLS
  • GLS1
  • KIAA0838
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~65 kDa observed; 73.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human neural cortex tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected GLS in 10 µg of human neural cortex tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ABN1466 04054839198977