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05-1938 Anti-G3BP1 Antibody, clone 14E5-G9

Anti-G3BP1 Antibody, clone 14E5-G9 is an antibody against G3BP1 for use in WB & IC.

Anti-G3BP1 Antibody, clone 14E5-G9: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling Research Sub-Category: RNA Metabolism & Binding Proteins, G-proteins Gene Symbol: G3BP, G3BP-1, HDH-VIII UniProt Number: Q13283
05-1938
100 µg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1938
DescriptionAnti-G3BP1 Antibody, clone 14E5-G9
Alternate Names
  • ATP-dependent DNA helicase VIII
  • GAP SH3 domain-binding protein 1
  • GAP binding protein
  • GTPase activating protein (SH3 domain) binding protein 1
  • Ras-GTPase-activating protein SH3-domain-binding protein
  • RasGAP-associated endoribonuclease G3BP
Background InformationG3BP1 (Ras-GTPase activating protein SH3-domain binding protein 1) directly associates with SH3 domains of GTPase activating proteins (GAPs). It was first discovered by its binding to RasGAP. G3BPs have been shown to be involved in a number of mitogenic signaling pathways. G3BP1 is also known to be over expressed in many human cancers (Kim, 2007) and its expression appears to be inversely correlated with PTEN expression (Kim, 2007). Interestingly, G3BP1, along with G3BP2, has been shown to bind to the C-terminus of p53, playing a crucial role in its activity (Kim, 2007). Furthermore, the G3BPs have two traditional RNA binding motifs.
References
Product Information
FormatPurified
Control
  • K562 cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-G3BP1 Antibody, clone 14E5-G9 is an antibody against G3BP1 for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human G3BP1.
EpitopeUnknown
Clone14E5-G9
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes G3BP1.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteProven to react with human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]
Gene Symbol
  • G3BP
  • G3BP-1
  • HDH-VIII
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

COFACTOR: Magnesium. Required for helicase activity.

SUBUNIT STRUCTURE: Binds to the SH3 domain of Ras GTPase-activating protein (RASA1) in proliferating cells. No interaction in quiescent cells Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP By similarity. Interacts with USP10, and may regulate it. Forms homodimers and oligomers.

SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytosol. Cell membrane. Nucleus. Note: Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells By similarity. Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.

TISSUE SPECIFICITY: Ubiquitous.

DOMAIN: The NTF2 domain mediates multimerization.

PTM: Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity By similarity. RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.

Arg-435 is dimethylated, probably to asymmetric dimethylarginine.

SEQUENCE SIMILARITIES: Contains 1 NTF2 domain.

Contains 1 RRM (RNA recognition motif) domain.
Molecular Weight52 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected G3BP1 on K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
05-1938 04053252506468

Documentation

Anti-G3BP1 Antibody, clone 14E5-G9 MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-G3BP1 Antibody, clone 14E5-G9 Certificati d'Analisi

TitoloNumero di lotto
Anti-G3BP1, clone 14E5-G9 - 3221518 3221518
Anti-G3BP1, clone 14E5-G9 - 3729374 3729374
Anti-G3BP1, clone 14E5-G9 - 3923750 3923750
Anti-G3BP1, clone 14E5-G9 - 4095783 4095783
Anti-G3BP1, clone 14E5-G9 -2537443 2537443
Anti-G3BP1, clone 14E5-G9 -2578180 2578180
Anti-G3BP1, clone 14E5-G9 -2650799 2650799
Anti-G3BP1, clone 14E5-G9 -2823944 2823944
Anti-G3BP1, clone 14E5-G9 -2847055 2847055
Anti-G3BP1, clone 14E5-G9 -2852316 2852316
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