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MABS1217 Anti-Fibulin-1 Antibody, clone 3D12.1

Detect Fibulin-1 using this Anti-Fibulin-1 Antibody, clone 3D12.1 validated for use in Western Blotting, Immunohistochemistry.

Anti-Fibulin-1 Antibody, clone 3D12.1 : MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: FBLN1, FIBL1, FBLN, PP213 UniProt Number: P23142
MABS1217
200 μg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1217
DescriptionAnti-Fibulin-1 Antibody, clone 3D12.1
Alternate Names
  • FIBL-1
  • Fibulin-1
Background InformationFibulin-1 (UniProt P23142; also known as FIBL-1) is encoded by the FBLN1 (also known as FIBL1, FBLN, PP213) gene (Gene ID 2192) in human. Fibulin-1 is an extracellular matrix protein that interacts with fibronectin and inhibits cell migration and invasion in a fibronectin-dependent manner. Research shows that the EGF-like domains 5 and 6 of fibulin-1 compete against syndecan-4 for binding the C-terminal heparin-binding domain (HepII) of fibronectin, resulting in a suppression of fibronectin-regulated cell adhesion and motility. Fibulin-1 also interacts with fibrinogen and regulates platelet adhesion to influence hemoglutination. In addition, fibulin-1 interacts with elastin and plays a role in the assembly of elastic fibers by mediating association between versican and fibrillin. Other fibulin-1 interactors include calumenin, extracellular matrix protein 1, angiogenin, and hemicentin. There exist four major fibulin-1 isoforms (A, B, C, and D) generated by alternative splicing, with isoforms C and D being the predominant forms. In addition, a smaller isoform of fibulin-1D, fibulin-1D′, is reported to localize in the cytosol and interacts with the intracellular domain of integrin β1. Fibulin-1 is initially produced with a signal peptide (a.a. 1-29), the removal of which yields the mature protein with three N-terminal Anaphylatoxin-like domains (a.a. 36-76, 77-111, 112-144), followed by eight EGF-like domains (a.a. 176-578 of isoform D), with EGF-like domains 5 & 6 mediating fibulin-1 self-association and fibronectin-binding. The C-terminal 137-amino acid in isoform D is truncated in isoform A, and replaced by a shorter sequence of 35 amino acids in isoform B and a sequence of 117 amino acids in isoform C.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Fibulin-1 using this Anti-Fibulin-1 Antibody, clone 3D12.1 validated for use in Western Blotting, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Fibulin-1 in human placenta and human breast tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to EGF-like domains 4-6 of human Fibulin-1.
EpitopeEGF-like domains 4-6.
Clone3D12.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with all four spliced isoforms of human fibulin-1 (UniProt P23142-1 to P23142-4).
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FBLN1
  • FIBL1
  • FBLN
  • PP213
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~77 kDa observed. 77.2 kDa (isoform D), 61.6 kDa (isoform A), 65.4 kDa (isoform B), and 74.4 kDa (Isoform C) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 4.0 µg/mL of this antibody detected Fibulin-1 in 10 µg of SH-SY5Y cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABS1217 04055977312942