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208912 Anti-Calreticulin Mouse mAb (FMC75)

Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.

Anti-Calreticulin Mouse mAb (FMC75): MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
208912
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, MkMMonoclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
208912-50UG Fiala di plastica 50 μg
Description
OverviewRecognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
Catalogue Number208912
Brand Family Calbiochem®
Application Data
Detection of human calreticulin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1) and Vero cells (lane 2). Primary antibody: Anti-Calreticulin Mouse mAb (FMC75) (Cat. No. 208912) (1:1000). Detection: chemiluminescence.
References
ReferencesNauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
Product Information
FormLiquid
FormulationIn PBS containing 50% glycerol and 0.09% sodium azide
Positive controlHuman Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting, Flow Cytometry Broquet, A.H., et al. 2003. J. Biol. Chem. 278, 21601. Immunocytochemistry Chen, A., et al. 2002. Biochim. Biophys. Acta 1589, 124. Morello, J.P., et al. 2000. J. Clin. Invest. 105, 887. Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150. Immunohistochemistry Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150.
Key Applications Enzyme Immunoassay
Flow Cytometry
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunohistochemistry
Application NotesEnzyme Immunoassay (0.1 µg/ml)
Immunoblotting (0.5 µg/ml, colorimetric; see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Flow Cytometry (see application references)
Application CommentsDoes not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena recombinant protein consisting of human calreticulin fused to maltose binding protein
ImmunogenHuman
CloneFMC75
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Monkey
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
208912-50UG 04055977219531

Documentation

Anti-Calreticulin Mouse mAb (FMC75) MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Calreticulin Mouse mAb (FMC75) Certificati d'Analisi

TitoloNumero di lotto
208912

Riferimenti bibliografici

Panoramica delle referenze
Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-December-2022 JSW
ApplicationEnzyme Immunoassay (0.1 µg/ml)
Immunoblotting (0.5 µg/ml, colorimetric; see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Flow Cytometry (see application references)
Application Data
Detection of human calreticulin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1) and Vero cells (lane 2). Primary antibody: Anti-Calreticulin Mouse mAb (FMC75) (Cat. No. 208912) (1:1000). Detection: chemiluminescence.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
BackgroundCalreticulin is a calcium binding protein found primarily in the ER and the sarcoplasmic reticulum, although it also has been detected in the nucleus and nuclear envelope. It has the conserved KDEL sequence at its C-terminus for retention in the ER. It contains both high and low affinity calcium binding sites, is involved in calcium storage and regulation. The calcium binding at the C-terminus of calreticulin has been postulated to be involved with regulating the interaction of KDEL proteins with the KDEL receptor. Calreticulin also acts as a molecular chaperone for protein folding, in concert with Grp78 and Grp94.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of human calreticulin fused to maltose binding protein
CloneFMC75
IsotypeIgG₁
Speciesnot E. coli, not bovine, not canine, not guinea pig, not hamster, human, monkey, not mouse, not rat, not salmon
Positive controlHuman Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
FormLiquid
FormulationIn PBS containing 50% glycerol and 0.09% sodium azide
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsDoes not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesNauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
Application referencesImmunoblotting, Flow Cytometry Broquet, A.H., et al. 2003. J. Biol. Chem. 278, 21601. Immunocytochemistry Chen, A., et al. 2002. Biochim. Biophys. Acta 1589, 124. Morello, J.P., et al. 2000. J. Clin. Invest. 105, 887. Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150. Immunohistochemistry Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150.