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CBL180-I Anti-CD62E [ELAM-1] Antibody, clone 1.2B6

Anti-CD62E [ELAM-1] Antibody, clone 1.2B6 is an antibody against CD62E [ELAM-1] for use in Immunohistochemistry, ELISA, Flow Cytometry, Western Blotting, Activity Assay.

Anti-CD62E [ELAM-1] Antibody, clone 1.2B6 : MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling Research Sub-Category: Adhesion (CAMs) Gene Symbol: SELE, ELAM, ELAM1, ESEL, SELP, GMRP, GMP140, GRMP, PSEL UniProt Number: P16581\P16109
CBL180-I
100 μg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, ELISA, FC, WB, ACTMPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL180-I
DescriptionAnti-CD62E [ELAM-1] Antibody, clone 1.2B6
Alternate Names
  • E-selectin
  • CD62 antigen-like family member E
  • CD62E
  • ELAM-1
  • Endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • Leukocyte-endothelial cell adhesion molecule 2
  • P-selectin
  • CD62 antigen-like family member P
  • CD62P
  • GMP-140
  • Granule membrane protein 140
  • LECAM3
  • Leukocyte-endothelial cell adhesion molecule 3
  • PADGEM
  • Platelet activation dependent granule-external membrane protein
  • Platelet alpha-granule membrane protein
Background InformationSelectins (CD62s) are single-chain transmembrane glycoproteins that function as cell adhesion molecules (CAMs) by interacting with a range of oligosaccharide structures including sialyl-Lewis X (sLeX) via their C-type lectin domain. All three known selectins (L-, E-, and P-selectin or CD62L CD62E, CD62P, respectively) share a similar overall domain structure that includes an N-terminal C-type lectin domain (calcium-dependent lectin domain), an EGF-like domain, a variable number of short consensus repeat (SCR) or Sushi domains (2, 6, and 9 for CD62L CD62E, CD62P, respectively), a transmembrane domain, and a cytoplasmic tail. The extracellular domains show a high homology among the three selectins, the transmembrane and cytoplasmic domains however are not conserved among the three. E-selectin (CD62E, ELAM-1) and P-selectin (CD62P, GMP-140, LECAM3, PADGEM) are mainly expressed on endothelial cells at inflammatory sites, whereas L-selectin (CD62L) is exclusively expressed on leukocytes.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-CD62E [ELAM-1] Antibody, clone 1.2B6 is an antibody against CD62E [ELAM-1] for use in Immunohistochemistry, ELISA, Flow Cytometry, Western Blotting, Activity Assay.
Key Applications
  • Immunohistochemistry
  • ELISA
  • Flow Cytometry
  • Western Blotting
  • Activity Assay
Application NotesELISA Analysis: A representative lot detected an induction of E-selectin expression on HUVECs by T-cells isolated from human PBMCs (Yarwood, H., et al. (2000). J. Leukoc. Biol. 68(2):233-242).
ELISA Analysis: A representative lot detected a time-dependent induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs, but not A549 cells (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Flow Cytometry Analysis: A representative lot specifically reacted with exogenously expressed human E- and P-, but not L-, selectin on the surface of transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Flow Cytometry Analysis: A representative lot detected an induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Western Blotting Analysis: A representative lot specifically reacted with E- and P-, but not L-, selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked the adhesion of HL-60 cells to E- or P-selectin transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked sialyl-Lewis X (sLeX) binding by E- and P-selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Immunohistochemistry Analysis: A representative lot detected ELAM-1 immunoreactivity in acetone-fixed frozen human skin tissue sections (Groves, R.W., et al. (1991). Br. J. Dermatol. 124(2):117-123).
Immunoprecipitation Analysis: A representative lot immunoprecipitated E-selectin and associated proteins from lysates of TNF-stimulated HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Biological Information
ImmunogenTNF-activated human umbilical vein endothelial cells.
EpitopeC-type lectin domain.
Clone1.2B6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThis antibody is specific to human antigen E-selectin (ELAM-1, 115 kDa) and P-selectin that are expressed on activated endothelial cells. The ligand is expressed on neutrophils and may function as an early part in neutrophil binding to the endothelium. This antibody inhibits neutrophil adhesion to E-selectin and P-selectin when used as F(ab')2 fragments. Although previously thought to recognize only human CD62E, later data (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560) showed that this antibody also recognizes human CD62P by binding to a common epitope on these members of the selectin family.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SELE
  • ELAM
  • ELAM1
  • ESEL
  • SELP
  • GMRP
  • GMP140
  • GRMP
  • PSEL
Purification MethodProtein G Purified
UniProt Number
Molecular Weight66 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human tonsil tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CD62E [ELAM-1] in human tonsil tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
CBL180-I 04055977312492