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MAB5230 Anti-CASK Antibody, clone K56A

Anti-CASK Antibody, clone K56A is an antibody against CASK for use in WB, IC, IH.

Anti-CASK Antibody, clone K56A: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Neuroscience Research Sub-Category: Synapse & Synaptic Biology Gene Symbol: CASK, LIN2, hCASK, EC 2.7.11.1 UniProt Number: O14936
MAB5230
100 µg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5230
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-CASK Antibody, clone K56A
References
Product Information
FormatPurified
PresentationPurified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CASK Antibody, clone K56A is an antibody against CASK for use in WB, IC, IH.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesWestern blot: 1 μg/mL

Immunocytochemistry: 0.5-1.0 μg/mL

Immunohistochemistry on 4% paraformaldehyde fixed tissue: 0.5- 1.0 μg/mL

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenCASK-GST fusion protein (amino acids 318-415).
CloneK56A
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityCASK. The immunogen protein represents a CASK-specific domain that lies between the calmodulin binding and PDZ domains. MAB5230 exhibits a specific reaction to the two alternatively spliced isoforms of CASK present in the mammalian CNS, the CASK-B or long (~900 amino acid) isoform present in rat brain membrane preparations, and the soluble CASK-A or short (~600 amino acid) isoform.CASK is a synapse-associated Ca2+/calmodulin-dependent protein kinase that also contains PDZ, SH3 and guanylate kinase domains. CASK is thought to play a critical role in synaptic vesicle exocytosis and synaptic junctions.
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe CASK gene encodes a calcium/calmodulin-dependent serine protein kinase that is a member of the membrane-associated guanylate kinase (MAGUK) protein family. MAGUKs are scaffolding proteins associated with intercellular junctions (Atasoy et al., 2007 [PubMed 17287346]).[supplied by OMIM]
Gene Symbol
  • CASK
  • LIN2
  • hCASK
  • EC 2.7.11.1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14936 # Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
COFACTOR: Magnesium (By similarity).
SIZE: 926 amino acids; 105123 Da
SUBUNIT: Binds WHRN and NRXN1 cytosolic tail. Interacts with CASKIN1, APBA1, LIN7(A/B/C) and L27 domain of DLG1 and isoform 2 of DLG4 (By similarity). CASK and LIN7 form two mutually exclusive tripartite complexes with APBA1 or CASKIN1 (By similarity). Interacts with FCHSD2. Interacts with TSPYL2. Part of a complex containing CASK, TRB1 and TSPYL2 (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity).
TISSUE SPECIFICITY: Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
DOMAIN: SwissProt: O14936 The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7 (By similarity). & The protein kinase domain mediates the interaction with FCHSD2.
SIMILARITY: In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Belongs to the MAGUK family. & Contains 1 guanylate kinase-like domain. & Contains 2 L27 domains. & Contains 1 PDZ (DHR) domain. & Contains 1 protein kinase domain. & Contains 1 SH3 domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots up to 6 months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MAB5230 04053252667756

Documentation

Anti-CASK Antibody, clone K56A MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-CASK Antibody, clone K56A Certificati d'Analisi

TitoloNumero di lotto
MOUSE ANTI-CASK -2646446 2646446
MOUSE ANTI-CASK -2683912 2683912
MOUSE ANTI-CASK MONOCLONAL ANTIBODY 2895739
MOUSE ANTI-CASK MONOCLONAL ANTIBODY 2926070
MOUSE ANTI-CASK MONOCLONAL ANTIBODY 3134705

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiApplicazioneCodice d'identificazione nel Pub Med
CASK deletion in intestinal epithelia causes mislocalization of LIN7C and the DLG1/Scrib polarity complex without affecting cell polarity.
Lozovatsky, L; Abayasekara, N; Piawah, S; Walther, Z
Molecular biology of the cell  20  4489-99  2009

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