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05-449 Anti-Bin1 Antibody, clone 99D

05-449
200 µg  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Replacement Information

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RIP, WB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-449
ReplacesMAB4131
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Bin1 Antibody, clone 99D
References
Product Information
FormatPurified
Presentation0.1M Tris-glycine, pH 7.4, containing 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-Bin1 Antibody, clone 99D is an antibody against Bin1 for use in IP, WB, IH.
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry
Biological Information
ImmunogenPartial fusion protein corresponding to amino acids 189-398 of human Bin1. Clone 99D
Cloneclone 99D
HostMouse
SpecificityBin1
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.
Gene Symbol
  • BIN1
  • AMPH2
  • amphiphysin-like
  • MGC10367
  • DKFZp547F068
  • AMPHL
  • SH3P9
Purification MethodProtein G Chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O00499 # May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
SIZE: 593 amino acids; 64699 Da
SUBUNIT: Heterodimer with AMPH. Binds SH3GLB1 (By similarity). Binds to SYNJ1 and DNM1 through its SH3 domain, and to clathrin through a region outside of the SH3 domain. Also binds AP2A2. Interacts with the N-terminal transactivation domain of MYC in a manner requiring the integrity of the conserved MYC box regions 1 and 2. Interacts with BIN2. Interacts with HCV NS5A through its SH3 domain.
SUBCELLULAR LOCATION: Isoform BIN1: Nucleus. & Isoform IIA: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
PTM: Phosphorylated by protein kinase C (By similarity).
DISEASE: "SwissProt: O00499 # Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers."
SIMILARITY: SwissProt: O00499 ## Contains 1 BAR domain. & Contains 1 SH3 domain.
Molecular Weight56kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on RIPA lysate of C2C12 cells.
Sales RestrictionsDue to license agreement restrictions, this product cannot be purchased for resale.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
05-449 04053252282911

Documentation

Anti-Bin1 Antibody, clone 99D MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Bin1 Antibody, clone 99D Certificati d'Analisi

TitoloNumero di lotto
Anti-Bin1, clone 99D (mouse monoclonal IgG1#954;) - 2166058 2166058
Anti-Bin1, clone 99D (mouse monoclonal IgG1κ) - 2441939 2441939
Anti-Bin1, clone 99D (mouse monoclonal IgG1k) - 1993899 1993899
Anti-Bin1, clone 99D (mouse monoclonal IgG1k) - 2239861 2239861
Anti-Bin1, clone 99D (mouse monoclonal IgG1k) - 2280418 2280418
Anti-Bin1, clone 99D (mouse monoclonal IgG1k) - 2344690 2344690
Anti-Bin1, clone 99D (mouse monoclonal IgG1κ) 2983268
Anti-Bin1, clone 99D (mouse monoclonal IgG1κ) 3150902
Anti-Bin1, clone 99D (mouse monoclonal IgG1κ) 3150916
Anti-Bin1, clone 99D (mouse monoclonal IgG1κ) 3106552

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiApplicazioneCodice d'identificazione nel Pub Med
Suppression of MEK/ERK signalling by Myc: role of Bin-1.
Telfer, JF; Urquhart, J; Crouch, DH
Cellular signalling  17  701-8  2004

Mostra il sommario
Immunohistochemistry15722194 15722194
A role for the putative tumor suppressor Bin1 in muscle cell differentiation
Wechsler-Reya, R. J., et al
Mol Cell Biol, 18:566-75 (1998)  1998

Immunoprecipitation, Immunoblotting (Western)9418903 9418903
The putative tumor suppressor BIN1 is a short-lived nuclear phosphoprotein, the localization of which is altered in malignant cells.
Wechsler-Reya, R, et al.
Cancer Res., 57: 3258-63 (1997)  1997

Mostra il sommario
9242458 9242458
BIN1 is a novel MYC-interacting protein with features of a tumour suppressor.
Sakamuro, D, et al.
Nat. Genet., 14: 69-77 (1996)  1996

Mostra il sommario
8782822 8782822