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MABC535 Anti-BIRC2/IAP-2, clone 1E1-1-10 Antibody

MABC535
100 µg  
Purchase on Sigma-Aldrich

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Replacement Information

Offerte speciali

Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC535
DescriptionAnti-BIRC2/IAP-2, clone 1E1-1-10 Antibody
Alternate Names
  • Baculoviral IAP repeat-containing protein 2
  • Inhibitor of apoptosis protein 2
  • IAP-2
  • mIAP-2
  • mIAP2
  • cIAP1
Background InformationBRC2/IAP-2, also known as IAP homolog B, Inhibitor of apoptosis protein 2 and Baculoviral IAP repeat-containing protein 2, and encoded by the gene BIRC2/API1/IAP2/MIHB/ RNF48, is a multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. BIRC2/IAP-2 also acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical pathways. Additionally, BIRC2/IAP-2 acts as an important regulator of innate immune signaling, and finally, BIRC2/IAP-2 plays an important role in the modulation of the cell cycle. BIRC2/IAP-21 is localized to nucleus except during apoptotic events. BIRC2/IAP-2 is expressed in fetal and adult tissues with a strong emphasis found in adult skeletal muscle, thymus, testis, ovary and pancreas with low or absent expression in blood and brain.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-BIRC2/IAP-2 antibody, clone 1E1-1-10 is an antibody against BIRC2/IAP-2 for use in western blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected BIRC2/IAP-2 in C2C12 cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in HeLa and Raji cell lysate (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.).

Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in MEF cell lysate (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.).

Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in stable cell lines expressing EYFP cIAP1 RING, or EYFP cIAP1 RING (Silke, J., et al. (2005). Proc Natl Acad Sci USA. 102(45):16182-16187.).
Biological Information
ImmunogenLinear peptide corresponding to the BIR 2 repeat region of mouse BIRC2/IAP-2.
EpitopeThis antibody recognizes the BIR 2 repeat region of BIRC2/IAP-2.
Clone1E1-1-10
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Birc2
  • Birc3
  • lap2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~65 kDa observed. The calculated molecular weight of this protein is 70 kDa; however, this protein has been observed at lower molecular weights (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.; Silke, J., et al. (2005). Proc Natl Acad Sci USA. 102(45):16182-16187.).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected BIRC2/IAP-2 in 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABC535 04053252955877

Documentation

Anti-BIRC2/IAP-2, clone 1E1-1-10 Antibody MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-BIRC2/IAP-2, clone 1E1-1-10 Antibody Certificati d'Analisi

TitoloNumero di lotto
Anti-BIRC2/IAP-2, clone 1E1-1-10 - Q2385778 Q2385778
Anti-BIRC2/IAP-2, clone 1E1-1-10 - 3989974 3989974
Anti-BIRC2/IAP-2, clone 1E1-1-10 - 4105673 4105673
Anti-BIRC2/IAP-2, clone 1E1-1-10 -2689657 2689657
Anti-BIRC2/IAP-2, clone 1E1-1-10 -2720120 2720120
Anti-BIRC2/IAP-2, clone 1E1-1-10 Monoclonal Antibody 3020539

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
Linear ubiquitination prevents inflammation and regulates immune signalling.
Gerlach, Björn, et al.
Nature, 471: 591-6 (2011)  2010

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Determination of cell survival by RING-mediated regulation of inhibitor of apoptosis (IAP) protein abundance.
Silke, John, et al.
Proc. Natl. Acad. Sci. U.S.A., 102: 16182-7 (2005)  2004

Mostra il sommario
16263936 16263936