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MABT379 Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1

This Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1 is validated for use in western blotting & IHC for the detection of Anoctamin-1/TMEM16A.

Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: ANO1, DOG1, ORAOV2, TAOS2 UniProt Number: Q5XXA6
MABT379
100 µg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT379
DescriptionAnti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1
Alternate Names
  • Anoctamin-1
  • Discovered on gastrointestinal stromal tumors protein 1
  • Oral cancer overexpressed protein 2
  • Transmembrane protein 16A
  • Tumor-amplified and overexpressed sequence 2
Background InformationDiscovered on gastrointestinal stromal tumors protein 1 (Dog1), also known as Anoctamin-1/TMEM16A, is a novel multi-pass membrane protein belonging to the anoctamin family. Anoctamin-1/TMEM16A functions as a Ca2+-activated Cl- channel and is a required component of tracheal development. Expression of Anoctamin-1/TMEM16A is elevated in the gastrointestinal stromal tumors (GISTs), KIT signaling-driven mesenchymal tumors of the GI tract. It is also expressed at more moderate levels in epithelia from foregut, kidney, lung, pancreas and salivary glands. Recent studies suggest that it may serve a roll as a diagnostic marker in the classification of GISTs.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1 is validated for use in western blotting & IHC for the detection of Anoctamin-1/TMEM16A.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Anoctamin-1/TMEM16A in human prostate cancer sections.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the N-terminal cytoplasmic domain of human Anoctamin-1/TMEM16A.
EpitopeN-terminal cytoplasmic domain
Clone5F8.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes isoforms 1 and 2 of Anoctamin-1 based upon immunogen design.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ANO1
  • DOG1
  • ORAOV2
  • TAOS2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~114 kDa and ~98 kDa observed. Three isoforms are known to exist at 114 kDa (isoform 1), 98 kDa (isoform 2), and 74 kDa (isoform 3).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC-3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Anoctamin-1/TMEM16A in 10 µg of PC-3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
MABT379 04053252955792