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16-293 Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 488

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor 488 is an antibody against Akt/PKB for use in FC, IC, IF & WB.

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 488: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: AKT1, RAC, PRKBA, MGC99656, RAC-ALPHA, RAC-PK-alpha, C-AKT, PKB, AKT UniProt Number: P31749
16-293
50 µg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RFC, ICC, IF, WBMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number16-293
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 488
Alternate Names
  • PKB
  • PKD-alpha
  • AKT1
  • Protein Kinase B
References
Product Information
FormatAlexaFluor®488
Quality LevelMQ100
Applications
ApplicationAnti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor 488 is an antibody against Akt/PKB for use in FC, IC, IF & WB.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Western Blotting
Biological Information
ImmunogenGST-fusion protein corresponding to residues 1-149 of human Akt1. Clone SKB1.
Cloneclone SKB1
HostMouse
SpecificityRecognizes Akt1/PKBα > Akt2/PKBβ (30%) > Akt3/PKBγ (weak to none) based on immunoblot analysis using 100ng recombinant fusion protein for each isoform.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Gene Symbol
  • AKT1
  • RAC
  • PRKBA
  • MGC99656
  • RAC-ALPHA
  • RAC-PK-alpha
  • C-AKT
  • PKB
  • AKT
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight60 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Immunocytochemistry and Flow Cytometry
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at 4°C from date of shipment
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
16-293 04053252273049

Documentation

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 488 Certificati d'Analisi

TitoloNumero di lotto
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor#174; 488 conjugate - 1994386 1994386
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor#174; 488 conjugate - 2145963 2145963
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor#174; 488 conjugate - JBC1854164 JBC1854164
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluorreg; 488 conjugate - D6D024 D6D024
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate (mouse monoclonal IgG) 3158056
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate (mouse monoclonal IgG) 3142394
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate - 3026849 3026849
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate -2532560 2532560
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate -2538632 2538632
Anti-Akt/PKB, PH Domain, clone SKB1, Alexa Fluor® 488 conjugate -2729446 2729446

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Alternative Format

Numero di catalogo Descrizione
05-591 Anti-Akt/PKB Antibody, PH Domain, clone SKB1

Categorie

Life Science Research > Antibodies and Assays > Primary Antibodies