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ABS102 Anti-AKAP 79 Antibody

Detect AKAP 79 using this Anti-AKAP 79 Antibody validated for use in WB.

Anti-AKAP 79 Antibody: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: AKAP5, AKAP79 UniProt Number: P24588
ABS102
100 µg  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS102
DescriptionAnti-AKAP 79 Antibody
Alternate Names
  • A-kinase anchor protein 5
  • AKAP-5
  • A-kinase anchor protein 79 kDa
  • AKAP 79
  • H21
  • cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Background InformationA-kinase anchoring protein 79 (AKAP 79) is a scaffolding protein that resides predominantly in excitatory neuronal synapses. AKAP 79 interacts with membrane-associated guanylate kinase (MAGUK) to be used by glutamate receptors. AKAP 79 may also be involved with SAP97 and other large signaling complexes. AKAP 79 is essential for forskolin-induced up-regulation of L-current.
References
Product Information
FormatAffinity Purified
Control
  • HEK293 cell lysate with overexpressed N-terminal Flag-AKAP79
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect AKAP 79 using this Anti-AKAP 79 Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human AKAP 79.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes AKAP 79 at the C-terminus.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq].
Gene Symbol
  • AKAP5
  • AKAP79
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.

SUBUNIT STRUCTURE: Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor.

SUBCELLULAR LOCATION: Membrane. Note: Associated with particulate fractions.

DOMAIN: RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

MISCELLANEOUS: The N-terminal region, which is highly basic, is required for interaction with calmodulin.

SEQUENCE SIMILARITIES: Contains 1 AKAP domain.
Molecular Weight~79 kDa observed.
AKAP79 migrates in WB with an apparent molecular weight of ~79 kDa. Uncharacterized bands appear at ~35 kDa and ~13 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate with overexpressed N-terminal Flag-AKAP79.

Western Blot Analysis: 1 µg/mL of this antibody detected AKAP 79 on 10 µg of HEK293 cell lysate with overexpressed N-terminal Flag-AKAP79.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ABS102 04053252420306