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ABE1870 Anti-AHCYL1 (IRBIT) Antibody

Anti-AHCYL1 (IRBIT) Antibody : MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: AHCYL1, DCAL, XPVKONA UniProt Number: O43865
ABE1870
100 µL  
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Tabella delle specifiche principali

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBChAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1870
DescriptionAnti-AHCYL1 (IRBIT) Antibody
Alternate Names
  • Putative adenosylhomocysteinase 2
  • AHCYL1(IRBIT)
  • AdoHcyase 2
  • DC-expressed AHCY-like molecule
  • IP(3)Rs binding protein released with IP(3)
  • IRBIT
  • S-adenosyl-L-homocysteine hydrolase 2
  • S-adenosylhomocysteine hydrolase-like protein 1
Background InformationPutative adenosylhomocysteinase 2 (EC 3.3.1.1; UniProt O43865; also known as AdoHcyase 2, DC-expressed AHCY-like molecule, IP(3)Rs binding protein released with IP(3), IRBIT, S-adenosyl-L-homocysteine hydrolase 2, S-adenosylhomocysteine hydrolase-like protein 1) is encoded by the AHCYL1 (also known as DCAL, PPP1R78, PRO0233, XPVKONA) gene (Gene ID 10768) in human. Originally identified by its binding and antagonist activity against teh intracellular calcium channel, IP3R, IRBIT is now known to interact with multiple targets and regulate diverse physiological events in addition to blocking IP3 access to IP3R. IRBIT complexes with ribonucleotide reductase (RNR) in a dATP-dependent manner and stabilizes RNR in its inactive, dATP-bound state. IRBIT also targets CaMKIIα kinase for inhibition by blocking calmodulin binding. Other known IRBIT targets include the ion transporter NBCe1-B, the Na+/H+ exchanger NHE3, the Cl- channel CFTR, the Cl−/HCO3− exchanger Slc26a6, the Fip1 component of the cleavage and polyadenylation specificity factor (CPSF) complex. IRBIT contains an S-adenosyl-l-homocysteine hydrolase (AHCY) homology domain in and an N-terminal extension containing a serine-rich region with multiple phosphorylation sites. Long-IRBIT, but not IRBIT, has an additional N-terminal appendage, termed the LISN (Long-IRBIT specific N-terminal) domain. IRBIT is highly conserved among species, and the human and mouse orthologs have 100% protein identity.
References
Product Information
FormatAffinity Purified
PresentationPurified chicken polyclonal antibody in PBS with 0.02% Sodium azide.
Quality LevelMQ100
Applications
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Tet-inducible short hairpin RNA-mediated AHCYL1 (IRBIT) downregulation in HeLa cells (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
Western Blotting Analysis: A representative lot detected immunoprecipitated AHCYL1 (IRBIT) from HeLa lysates by Western blotting (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
Western Blotting Analysis: A representative lot detected the association of His-tagged human AHCYL1 (IRBIT) with human IP3R1(1-604) GST fusion protein by Western blotting analysis of the affinity pull-down obtained with Glutathione beads (Arnaoutov, A., and Dasso, M. (2014). Science 345(6203):1512-1515).
Biological Information
ImmunogenRecombinant protein corresponding to human AHCYL1(IRBIT).
ConcentrationPlease refer to lot specific datasheet.
HostChicken
SpecificityExpected to react with both spliced isoforms reported by UniProt (O43865-1 and O43865-2).
IsotypeIgY
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • AHCYL1
  • DCAL
  • XPVKONA
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~70 kDa observed. 59.0 kDa (isoform 1) and 53.8 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected AHCYL1 (IRBIT) in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
ABE1870 04055977311785

Documentation

Anti-AHCYL1 (IRBIT) Antibody MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-AHCYL1 (IRBIT) Antibody Certificati d'Analisi

TitoloNumero di lotto
Anti-AHCYL1(IRBIT) -Q2635129 Q2635129

Riferimenti bibliografici

Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
Enzyme regulation. IRBIT is a novel regulator of ribonucleotide reductase in higher eukaryotes.
Arnaoutov, A; Dasso, M
Science (New York, N.Y.)  345  1512-5  2014

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