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SCR139 Animal Free Collagenase/Dispase Blend I

Animal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.

Animal Free Collagenase/Dispase Blend I : MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Key Applications: Cell Harvesting, Cell Culture
SCR139
50mg  
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Key ApplicationsSource
Harv, CULTIsolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2015 certified enviroment.
Description
Catalogue NumberSCR139
DescriptionAnimal Free Collagenase/Dispase Blend I
Alternate Names
  • MMP
  • Matrix metalloprotease
  • Dispase
  • Neutral Protease
Background InformationCrude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types I, II, III, and IV or chromatographically purified collagenase. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. EMD Millipore’s Animal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired. The product is prepared and purified from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens, such as BSE,TSE and other mammalian viruses, must be prevented.
References
Product Information
PresentationProduct is diafiltered with water, filtered through a 0.22 micron membrane and lyophilized in vials.
ActivityCollagenase Activity: ≥12,500 units per vial
Caseinase Activity: ≥1000 caseinase units/mg
Clostripain Activity: ≤5.0 clostripain units/mg
Tryptic Activity: ≤0.5 trypic units/mg
Quality LevelMQ200
Applications
ApplicationAnimal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.
Key Applications
  • Cell Harvesting
  • Cell Culture
Biological Information
SourceIsolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2015 certified enviroment.
Physicochemical Information
ActivityCollagenase Activity: ≥12,500 units per vial
Caseinase Activity: ≥1000 caseinase units/mg
Clostripain Activity: ≤5.0 clostripain units/mg
Tryptic Activity: ≤0.5 trypic units/mg
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.
Packaging Information
Material Size50mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
SCR139 04053252982682

Documentation

Animal Free Collagenase/Dispase Blend I MSDS

Titolo

Scheda di sicurezza (MSDS) 

Animal Free Collagenase/Dispase Blend I Certificati d'Analisi

TitoloNumero di lotto
Animal Free Collagenase/Dispase - VP1907051 VP1907051
Animal Free Collagenase/Dispase Blend I - VP1903215 VP1903215
Animal Free Collagenase/Dispase Blend I Purified Enzyme Blend VP1807102
Animal Free Collagenase/Dispase Blend I Purified Enzyme Blend VP1704030

Scheda tecnica

Titolo
Animal Free Collagenase/Dispase Blend I Purified Enzyme Blend