Agarose-Based Affinity Purification
Agarose based IP and antibody purification
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| Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for IP & Antibody Purification |
Protein A and Protein G are proteins of microbial origin that bind specifically but differently to mammalian immunoglobulins. When coupled to agarose, they provide an efficient tool for purification and immunoprecipitation (IP) of antibodies. Protein A agarose binds to the Fc region of IgG from a variety of species and can be used to purify classes, subclasses, and fragments of Igs and to isolate immune complexes. Protein G agarose is useful for binding to Igs that do not bind Protein A and can be used for antibody IP for purifying Igs and IgG fractions. Combining Protein A and Protein G agarose is a good strategy for exploiting the power of both Ig binding affinities in a single reagent.
Features & Benefits
- Agarose beads with either immobilized Protein A, Protein G, or a mixture of Protein A and G for optimal affinity
- Compatible with Amicon® Pro purification system
- High binding capacity and low ligand leaching
- Two convenient formats, one for purification in column or batch mode and the other is a ready to-use suspension containing BSA for IP applications
Applications
Immunoprecipitation, Affinity Purification, Antibody Purification
Product Performance
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