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Kit per la preparazione di DNA plasmidico e di BAC nel formato a 96 pozzetti

Simply clear, concentrate, wash, and recover

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FAQ

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What is holdup volume of a MultiScreen Plate used with the Montage Kits?The holdup volume for the MultiScreen plate is between 5-20 microliters.
When using the Montage kits how can I tell when filtration in the MultiScreen plate is complete?Filtration is complete when the individual wells of the MultiScreen plate are empty and the membranes appear shiny.
The two sections of the MultiScreen plates used in the Montage kits are separating under vacuum. Why?The most common reason for this problem is the lack of a MultiScreen underdrain support grid in the vacuum manifold. A MultiScreen support grid is required for proper functioning of the MultiScreen plate with any vacuum manifold.
The total yields with the Montage Plasmid Miniprep-96 kit seems to be less then with competitive products. Why?The Montage kit uses less culture volume (1ml vs. 1.3 ml) than most competitive kits. However the relative yield is equivalent.
What is the total processing time for plasmid preparation using the Montage kits?Processing time depends on viscosity of sample, number of manifolds, familiarity with protocol, temperature. (Also, need vacuum capable of 24 in Hg or 812.7 millibar, 609.6 torr.)
Can the culture block in the Montage 96 Plasmid prep be autoclaved?Yes.
What is the 260/280 assessment ratio when using the Montage Plasmid Kit?The 260/280 reading is 1.8-2.0. This signifies and extremely high level of purity.
Can I use this kit in centrifugal mode?Use of the Montage Plasmid Miniprep-96 Kit in a centrifugal protocol is not recommended.
I'm using your two plate plasmid prep protocol and the wells are overflowing when I transfer the cleared lysate to the FB plate. What am I doing wrong?Overflowing can result from any of the following:
  • Using too much alkaline lysis and/or binding solutions.
  • Not removing all of the cell culture medium after pelleting cells. Tap the deep well block harder after decanting .
  • Cell pellet may be too large, as the result of overgrowth of the culture. Use a less rich medial like 2xLB.
I have observed RNA contamination of my plasmid preps. Why?Failure to add RNase A to solution 1 will often result in RNA contamination in your DNA miniprep.
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