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SCC300 3dGRO™ Human iPSC Derived Colon Organoids

Highly characterized cryopreserved human colon intestinal organoids derived from integration-free human induced pluripotent stem cells (iPSCs).

SCC300
≥ 1500 organoids/vial  
Purchase on Sigma-Aldrich

Panoramica

Replacement Information
Description
Catalogue NumberSCC300
Description3dGRO™ Human iPSC Derived Colon Organoids
Alternate Names
  • Human intestinal organoids
  • Human colonic organoids
  • Human gut organoids
Background InformationHuman intestinal organoids are self-organizing, 3D structures that can be expanded in long-term culture and differentiate into intestinal epithelial cell types. Epithelial intestinal organoids, often referred to as enteroids or “mini-guts”, maintain the physiological characteristics of the gastrointestinal system and have been a useful cell culture tool to model intestinal development and disease including the study of colon cancer, celiac disease, inflammatory bowel diseases and host microbiome interactions. Organoids can be propagated from patient biopsies and from embryonic (ES) and induced pluripotent stem cells (iPS cells).

3dGRO™ Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550). Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids (Figure 1.). The organoids express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B. 3dGRO™ human iPSC derived colon organoids can be propagated long-term by passaging every 10-12 days using 3dGRO™ Human Colon Organoid Expansion Medium (Cat. No. SCM304).
References
Product Information
Applications
ApplicationHighly characterized cryopreserved human colon intestinal organoids derived from integration-free human induced pluripotent stem cells (iPSCs).
Key Applications
  • Cell Culture
  • Stem Cell Culture
Biological Information
Cell Line Type
  • Organoid
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1500 organoids.
• Mycoplasma: Negative
• Genotyped by STR analysis: Pass
• Human species verified
• Organoids are negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cell viability test: Pass
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions3dGRO™ Human iPSC Derived Colon Organoids should be stored in liquid nitrogen.
Packaging Information
Material Size≥ 1500 organoids/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
SCC300 04054839989728