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ECM675 3D Collagen Culture Kit

ECM675
1 kit  
Purchase on Sigma-Aldrich

Offerte speciali

Panoramica

Tabella delle specifiche principali

Key Applications
Enzyme Assays
Description
Catalogue NumberECM675
Brand Family Chemicon®
Trade Name
  • Chemicon
Description3D Collagen Culture Kit
OverviewCell migration is a fundamental function of normal cellular processes, including cell proliferation, and migration. Cell behavior in vitro is typically examined in a 2D environment; however, in the body, cells exist in a three-dimensional extracellular matrix environment rich in type I collagen. In fact, cells cultured in 3D matrices better reflect in vivo cell physiology when compared to traditional 2D systems.

CHEMICON (EMD Millipore) has developed a three-dimensional cell culture system that closely simulates the collagen-rich extracellular matrix of normal tissue. This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.

As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.

For Research Use Only; Not for use in diagnostic procedures
Materials Required but Not Delivered· Tissue Culture Dishes

· Pipettes and Sterile Tips

· Microcentrifuge Tubes, Sterile

· 37oC Incubator

· Inverted Light Microscope

· Bacterial Collagenase
Product Information
Components
  • Collagen Solution - (Part No. 90135) One bottle - 10 mL
  • 5X RPMI Medium- (Part No. 90134) One bottle - 2.5 mL
  • 5X M199 Medium- (Part No. 90133) One bottle - 2.5 mL
  • 5X DMEM Medium- (Part No. 90137) One bottle - 2.5 mL
  • 5X PBS with Phenol Red- (Part No. 90136) One bottle - 2.5 mL
  • Neutralization Solution- (Part No. 90138) One vial - 0.5 mL
PresentationCollagen solution is supplied in 6mM acetate buffer at ~ pH7.0.
Quality LevelMQ100
Applications
Key Applications
  • Enzyme Assays
Application NotesThis 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.

As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
Biological Information
Species Reactivity
  • Human
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore kit materials at 4°C up to their expiration date.
Packaging Information
Material Size1 kit
Global Trade Item Number
Numero di catalogo GTIN
ECM675 04053252516139