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344826 α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli

This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides.

α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli: MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.

Sinonimi: α-L-Fucoside Fucohydrolase

344826
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Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
344826-50MIU Fiala di plastica 50 miu
Description
OverviewRecombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.
Note: 1 mU = 1 milliunit.
Catalogue Number344826
Brand Family Calbiochem®
Synonymsα-L-Fucoside Fucohydrolase
References
ReferencesZheng, Q., et al. 2002. J. Biol. Chem. 277, 39823.
Product Information
CAS number9037-65-4
Activity≥1.0 unit/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.
EC number3.2.1.51
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥1.5 units/mg protein
Physicochemical Information
ContaminantsProteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numero di catalogo GTIN
344826-50MIU 04055977214826

Documentation

α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli MSDS

Titolo

Scheda di sicurezza (MSDS) 

α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli Certificati d'Analisi

TitoloNumero di lotto
344826

Riferimenti bibliografici

Panoramica delle referenze
Zheng, Q., et al. 2002. J. Biol. Chem. 277, 39823.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-October-2007 JSW
Synonymsα-L-Fucoside Fucohydrolase
DescriptionRecombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium meningosepticum) catalyses the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Recommended reaction conditions
50 mM sodium phosphate buffer, pH 5.0.
CAS number9037-65-4
EC number3.2.1.51
ContaminantsProteases: none detected
Specific activity≥1.5 units/mg protein
Activity≥1.0 unit/ml
Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesZheng, Q., et al. 2002. J. Biol. Chem. 277, 39823.