ProteoExtract® Kits - Albumin/lgG Depletion |
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Efficient removal of albumin from human serum samples
Human plasma (35 ml) was processed with ProteoExtract® Albumin Removal Kit. From each fraction, 15 mg protein was separated on SDS-PAGE and visualized by Coomassie™ blue staining. Densitometric analysis of stained bands demonstrated that more than 80% of albumin was removed from the serum using the ProteoExtract® Albumin Removal Kit.
Key - M: Marker proteins, P: Human plasma, F: Flow-through, E: Eluate fraction
Human plasma (35 ml) was processed with ProteoExtract® Albumin Removal Kit. From each fraction, 15 mg protein was separated on SDS-PAGE and visualized by Coomassie™ blue staining. Densitometric analysis of stained bands demonstrated that more than 80% of albumin was removed from the serum using the ProteoExtract® Albumin Removal Kit.
Key - M: Marker proteins, P: Human plasma, F: Flow-through, E: Eluate fraction
Efficient and reproducible removal of albumin and IgG from serum samples
Using the ProteoExtract® Albumin/IgG Removal Kit, 35 mL human serum was processed, analyzed on 10% SDS-PAGE, and visualized by Coomassie™ blue staining. Densitometric analysis of stained bands of the eluate (E) fractions demonstrates that 88% ±5% albumin and 90% ±4% immunoglobulin are removed from the crude serum (S) using the ProteoExtract® Albumin/IgG Removal Kit.
Key - M: Markers, S: Crude human serum, F: Flow through: depleted serum in four replicates, E: Eluate fraction: proteins eluted from depleting columns in four replicates
Using the ProteoExtract® Albumin/IgG Removal Kit, 35 mL human serum was processed, analyzed on 10% SDS-PAGE, and visualized by Coomassie™ blue staining. Densitometric analysis of stained bands of the eluate (E) fractions demonstrates that 88% ±5% albumin and 90% ±4% immunoglobulin are removed from the crude serum (S) using the ProteoExtract® Albumin/IgG Removal Kit.
Key - M: Markers, S: Crude human serum, F: Flow through: depleted serum in four replicates, E: Eluate fraction: proteins eluted from depleting columns in four replicates
High capacity columns specifically designed for the rapid and efficient removal of >80% of albumin from body fluid samples, either on an LC-instrument or manually (attached to a syringe). Removal of these high abundance proteins allows enhanced detection of low abundance proteins. ProteoExtract Albumin Removal Kit, Maxi
High capacity columns specifically designed for the rapid and efficient removal of >80% of albumin (column 1 containing albumin-affinity resin) and IgG (column 2 containing immobilized protein A) from body fluid samples. ProteoExtract® Albumin/IgG Removal Kit
High capacity, reusable columns specifically designed for the rapid and efficient removal of >80% of albumin (column 1 containing albumin-affinity resin) and IgG (column 2 containing immobilized protein A) from body fluid samples. ProteoExtract® Albumin/IgG Removal Kit, Maxi
High capacity, reusable columns specifically designed for the rapid and efficient removal of >80% of albumin (column 1 containing albumin-affinity resin) and IgG (column 2 containing immobilized protein A) from body fluid samples.