Microcon® Centrifugal Filters

Request Information

---

Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel® membrane, Microcon® filters offer:

• Typical recoveries of >95%, even for dilute solutions
• Reverse spin to maximize recovery, even in the smallest samples
• Convenient storage of filtrate or concentrated sample in standard microfuge tube
• Concentration factors up to 100X

Microcon® DNA Fast Flow PCR Grade Filter

Working with low concentrations of DNA? Purifying DNA prior to PCR amplification? Use the PCR grade Microcon® ultrafiltration device. The dual cycle Ethylene Oxide (EtO) treated Microcon® DNA Fast Flow PCR grade devices enable centrifugal concentration at a similar rate as the Microcon® DNA Fast Flow devices, but the EtO treatment greatly reduces the risk that no uncontrolled contaminating nucleic acids enter the analysis process.

Microcon® DNA Fast Flow Filter

Optimized for the concentration and recovery of genomic DNA with SDS buffer. The low nonspecific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.

Features:

• High recovery for small volumes with reverse spin (concentration factor <20x) li="">Low-binding Ultracel® membrane
• Fast processing

Sample applications:

• Recovery of genomic DNA for forensic applications
• Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
• Removal of primers from amplified DNA
• Easy filtrate recovery, exploiting the flat membrane design

• Click for Applications Guidelines

  
  Use the following table to choose the correct Microcon® device for your application.
  
  

  	Microcon® Device
  Application	10K	30K	DNA Fast Flow
  Peptide and growth factor concentrationPeptide and growth factor concentration	•
  Protein concentration and desalting of columns eluates	•	•
  Protein concentration before electrophoresis or other assays	•	•
  Protein removal prior to HPLC	•	•
  Purification of macromolecular components found in tissue culture extracts and cell lysates	•	•
  Concentration of biological samples (antigens, antibodies, enzymes)		•
  Concentration of gDNA with or without SDS buffer		•	•
  Concentration and desalting of nucleic acids (single-or double-stranded)	•	•	•
  Removal of labeled nucleotides	•	•	•
  Removal of labeled amino acids	•	•	•
  Removal of primers from amplified DNA		•	•
  Removal of linkers prior to cloning		•	•

  
  

• Order Microcon® Filters

• Click for Specifications

  
  

  Maximum initial sample volume	0.5 mL (500 μL)
  Typical final concentrate volume	5–50 μL
  Maximum relative centrifugal force
  Microcon® 10K devices	14,000 × g
  Microcon® 30K devices	14,000 × g
  Microcon® DNA Fast Flow devices	500 × g
  Recovery spin	1,000 × g
  Active membrane area	0.32 cm2
  Hold-up volume	≤ 10 μL
  Dimensions
  Diameter	12.3 mm (0.5 in.)
  Length (filter device and tube in concentration mode)	45.0 mm (1.8 in.)
  Length (filter device and tube in recovery mode)	48.2 mm (1.9 in.)
  Materials of Construction
  Membrane	Ultracel® low binding regenerated cellulose
  Device top	Polycarbonate
  Membrane support base	Acetal
  Filtrate/concentrate tube	Polypropylene
  O-ring	Medical-grade silicone rubber