D-Tube™ Dialyzers |
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The disposable, single-use tubes require no syringes, centrifuge, or laborious steps to manipulate small sample volumes. The sample is added and removed using a standard laboratory pipette. D-Tube™ Dialyzers are available with molecular weight cut-offs from 3.5 to 14 kDa and are designed with six volume capacities:
- mini (10–250 μL)
- midi (50–800 μL)
- maxi (100– 3000 μL)
- and mega (10, 15, and 20 μL)
| Sample Type | Method | Typical Recovery |
|---|---|---|
| Sample in Solution | Dialysis | > 89% |
| DNA or RNA in agarose gel slice | Electroelution | > 90% |
| Oligonucleotides, DNA, or RNA in polyacrylamide gel slice | Electroelution | > 90% |
| Protein polyacrylamide gel slice | Electroelution | 60% |
Advantages:
- 89% Sample Recovery
- Low binding membrane and housing enhance sample recovery
- Reliable and Easy to Use
- Secure design prevents sample loss due to leaks — no knots or clamps to loosen and leak
- Easy to open and close with a screw cap
- Rigid frame permits smooth sample withdrawal of submilliliter volumes — removing every last drop is easy
- Convenient Sample Loading
- No need to use a syringe to load or remove samples. Simply load your sample with standard pipette tip
- Floating racks fit most standard beakers to hold devices in exchange buffer
- D-Tube™ dialyzers can also be used to electroelute samples from agarose or acrylamide
D-Tube™ Dialyzers provide gentle, efficient desalting and excellent protein recovery. A bovine serum albumin (BSA) solution in 2M NaCl was dialyzed against water while water conductivity was monitored. After 100 minutes, conductivity of the water stopped increasing, and it was replaced with fresh water. After 5 hours, 89% of the protein was recovered, and 99.9% of the salt had been removed.