Samplicity® G2 System: No Cross-Contamination |
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No Cross-Contamination Between Filter Positions on the Samplicity® G2 System
In row 1, filter positions 1, 3, 5, and 7 were used to filter fluorescein-containing samples. In row 2, filter positions 2, 4, 6, and 8 were used to filter the fluorescein-containing samples.*| Fluorescence Intensity | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Filter Position | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
| Unit 1 | Row 1 | >100,000 | 39 | >100,000 | 41 | >100,000 | 40 | >100,000 | 34 |
| Unit 1 | Row 2 | 35 | >100,000 | 44 | >100,000 | 36 | >100,000 | 35 | >100,000 |
| Unit 2 | Row 1 | >100,000 | 34 | >100,000 | 36 | >100,000 | 37 | >100,000 | 38 |
| Unit 2 | Row 2 | 34 | >100,000 | 36 | >100,000 | 30 | >100,000 | 35 | >100,000 |
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* Samples containing 0.005% fluorescein in 80:20 (v/v) acetonitrile:water were filtered using every other filter position in the Samplicity® G2 system, through 0.45 µm hydrophilic PTFE 33 mm Millex® filters. Similar results were obtained with 0.45 µm hydrophilic PES and PVDF 33 mm Millex® filters. Fluorescence signal was measured by spectrophotometry. Note: The signal was completely saturated on the filter positions in which the original samples were added. Values are given as an approximation of being greater than what is detectable with the spectrophotometer (≤100,000 fluorescence units). |
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