| Method | ISO 21872 | FDA BAM Chapter 9 |
|---|---|---|
| 1st selective enrichment | Alkaline Saline Peptone Water 37 ± 1°C or 41.5 ±1°C (fresh products); 6 ± 0.5 h |
Alkaline Peptone Water 35 ± 2°C; 7 ± 1 h (18-21 h for processed foods) / 42±0.2°C; 18-21 h for raw oysters. |
| 2nd selective enrichment | Alkaline Saline Peptone Water 41.5 ±1°C; 18 ± 1 h | N/A |
| Plating |
CBS Agar, 37 ±1°C; 21 ± 3 h |
TCBS Agar, 35 ± 2°C; 21 ± 3 h |
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Vibrio cholerae is a strictly aqueous organism. Brackish and marine waters are natural environments of Vibrio cholerae O1 or O139, the etiologic agents of the disease cholera. The main route of transmission is fecal-oral, usually indirectly via polluted water supplies or irrigation water. Another common source is raw or undercooked shellfish.
Merck offers a considerable selection of products for the enrichment, detection and isolation of Vibrio cholerae. The range includes dehydrated culture media in a unique and easy-to-use granulated format. These meet the highest industry standards for performance as laid out in ISO 11133.
