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69658 pLysE DNA

69658
  
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      This product has been discontinued.





      pLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into the BamH I site of pACYC184 (1). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to the tet promoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from the tet promoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.

      This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
      Catalogue Number69658
      Brand Family Novagen®
      References
      References1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.
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      Toxicological Information
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      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Specifications
      Global Trade Item Number
      Référence GTIN
      69658 0

      Documentation

      pLysE DNA FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      pLysE DNA Certificats d'analyse

      TitreNuméro de lot
      69658

      Références bibliographiques

      Aperçu de la référence bibliographique
      1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.

      Citations

      Titre
    • Mark S. McClain and Timothy L. Cover. (2007) Functional analysis of neutralizing antibodies against Clostridium perfringens epsilon-toxin. Infection and Immunity 75, 1785-1793.
    • Carte des vecteurs

      Titre
      TB107 pLysS and pLysE Plasmid Map

      Séquence du vecteur

      Titre
      pLysE Sequence

      Produits & Applications associés

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      Catégories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors