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71733 pIEx/Bac™-5 3C/LIC Vector Kit

pIEx/Bac™-5 3C/LIC Vector Kit : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
71733
  
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      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The pIEx/Bac™-5 3C/LIC vector carries an N-terminal GST•Tag™ coding sequence followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ_GP, and is active at low temperatures. The 3C/LIC cloning site is followed by an optional C-terminal thrombin recognition site and a Strep•Tag® II coding sequence. This 3C/LIC vector is ready for rapid, directional cloning of PCR-amplified DNA, without restriction enzyme digestion or ligation. The pIEx/Bac vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus.
      Catalogue Number71733
      Brand Family Novagen®
      References
      Product Information
      •20 µg (40 µl) 0.5 µg/µl pIEx/Bac-5 3C/LIC DNA
      •50 µlT4 DNA Polymerase Buffer
      •5 µg (10 µl) 0.5 µg/µl pIEx/Bac 3C/LIC Positive Expression Control
      •25 UT4 DNA Polymerase, LIC-qualified
      •100 µl100 mM DTT
      •40 µl25 mM dATP
      •50 µl25 mM EDTA
      •1.5 ml Nuclease-free Water
      •1 pkgNovaBlue GigaSingles Competent Cells
      •2 mlSOC Medium
      •10 µlTest Plasmid
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 22-36/37/38

      Harmful if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Harmful
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      71733 0