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SCC421 VCaP-EnzR Human Enzalutamide Resistant Prostate Cell Line

VCaP-EnzR Human Enzalutamide Resistant Prostate Cell Line is a valuable research model for studying castration resistant prostate cancer

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SCC421
≥1X10⁶ cells/vial   
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      Replacement Information
      Description
      Catalogue NumberSCC421
      DescriptionVCaP-EnzR Human Enzalutamide Resistant Prostate Cell Line
      Alternate Names
      • RRID:CVCL_RA58
      Background InformationProstate cancer (PCA) is the most frequently diagnosed cancer among men in the USA, with over 150 000 new cases expected annually. Approximately 6% become metastatic, with a 5-yr survival rate of only 29%. Metastatic lesions form most frequently in bone. Since the 1940s, the standard treatment for advanced PCA has been surgical or chemical castration targeting the androgen receptor (AR) signaling axis. Most treated patients, however, develop resistance and become androgen independent, which contributes to disease progression and poor prognosis, delineating a critical need to identify novel targetable pathways mediating resistance (1). The scientific investigation of PCA tumorigenesis and experimental therapies has been limited to a few of cell- and animal-based model systems.

      Source:
      Xenografted into SCID mice before propagated in cell culture, the parental PCA cell line VCaP derives from a vertebral metastatic lesion of a hormone refractory prostate cancer patient. Successfully cultured for over 50 passages in vitro, VCaP is considered an immortalized cell line (2). VCaP-EnzR is an enzalutamide resistant (EnzR) derivative of VCaP, developed by prolonged in vitro culture with enzalutamide, a second-generation AR antagonist efficacious in the treatment of castration resistant prostate cancer. VcaP-EnzR is a valuable research tool for studying castration resistant prostate cancer (3).

      Biosafety Level 2
      VCaP-EnzR cells contain mouse xenotropic retrovirus Bxv-1 and should be handled under biosafety level 2. The parental VCaP prostate cancer cell line is known to produce the mouse xenotropic rerovirus Bxv-1, which was likely acquired by the cells during their xenotransplantation in mice.

      References:
      1. Kelly SP et al. (2018) Past, current and future incidence rates and burden of metastatic prostate cancer in the United States. Eur Urol Focus 4(1):121–127.
      2. Korenchuk S et al. (2001) VcaP, a cell-based model system of human prostate cancer. In Vivo 15(2):163-168.
      3. Kregel S et al. (2016) Acquired resistance to the second-generation androgen receptor antagonist enzalutamide in castration-resistant prostate cancer. Oncotarget 7(18):26259-26274.
      References
      Product Information
      Applications
      ApplicationVCaP-EnzR Human Enzalutamide Resistant Prostate Cell Line is a valuable research model for studying castration resistant prostate cancer
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCC421 04065266024135