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58656 Thermolysin, Geobacillus stearothermophilus

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
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      Aperçu

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Native thermolysin from Geobacillus stearothermophilus. Zinc metalloendoproteinase with specificity toward isoleucine, leucine, methionine and valine. Inhibitors include EDTA, 1,10-phenanthroline, phosphinates, and phosphoamidates. Not inhibited by aprotinin or other serine protease inhibitors. Contains a zinc atom essential for activity and four Ca2+ essential for thermal and conformational stability. Note: 1 KU = 1000 units.
      Note: 1 KU = 1000 units.
      Catalogue Number58656
      Brand Family Calbiochem®
      References
      ReferencesPfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523.
      Matsubara, H. 1970. Methods Enzymol. 19, 642.
      Ohta, Y., et al. 1966. J. Biol. Chem. 241, 5919.
      Product Information
      CAS number37246-64-3
      Activity≥7,000,000 PU/g dry weight

      PU = protease unit
      Unit of DefinitionOne protease unit is defined as the amount of the enzyme that liberates the non-proteinous digestion product from milk casein at final concentration of 0.5% to give Folin's color equivalent to 1 µg of tyrosine per min at the early stage of the reaction at 35°C, pH 7.2.
      EC number3.4.24.2
      FormLyophilized
      FormulationLyophilized solid containing calcium acetate, sodium acetate, 3X crystallized.
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSXH7500000
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      58656 0

      Documentation

      Thermolysin, Geobacillus stearothermophilus FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Thermolysin, Geobacillus stearothermophilus Certificats d'analyse

      TitreNuméro de lot
      58656

      Références bibliographiques

      Aperçu de la référence bibliographique
      Pfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523.
      Matsubara, H. 1970. Methods Enzymol. 19, 642.
      Ohta, Y., et al. 1966. J. Biol. Chem. 241, 5919.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-June-2015 JSW
      DescriptionNative thermolysin from Geobacillus stearothermophilus. A highly thermostable zinc metallo-endoproteinase that exhibits specificity toward isoleucine, leucine, methionine, and valine. Inhibitors include EDTA, 1,10-phenathroline, phosphinates, and phosphoamidates. Not inhibited by aprotinin or other serine protease inhibitors. Contains a zinc atom essential for activity and four Ca2+ essential for thermal and conformational stability. Has an optimal pH in the range 7.0-9.0.
      FormLyophilized
      FormulationLyophilized solid containing calcium acetate, sodium acetate, 3X crystallized.
      Concentration Label Please refer to vial label for lot-specific concentration
      CAS number37246-64-3
      RTECSXH7500000
      EC number3.4.24.2
      Activity≥7,000,000 PU/g dry weight

      PU = protease unit
      Unit definitionOne protease unit is defined as the amount of the enzyme that liberates the non-proteinous digestion product from milk casein at final concentration of 0.5% to give Folin's color equivalent to 1 µg of tyrosine per min at the early stage of the reaction at 35°C, pH 7.2.
      SolubilityReconstitute in 42% glycerol adjusted to pH 8.0 with 0.01 N NaOH, containing 0.005% Triton® X-100 detergent.
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Harmful
      Merck USA index14, 9286
      ReferencesPfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523.
      Matsubara, H. 1970. Methods Enzymol. 19, 642.
      Ohta, Y., et al. 1966. J. Biol. Chem. 241, 5919.

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      Catégories

      Life Science Research > Proteins and Enzymes > Other Enzymes