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58346 Tetracycline, Hydrochloride - CAS 64-75-5 - Calbiochem

CAS 64-17-5 is a broad-based antibiotic agent that blocks protein synthesis by inhibiting binding of aminoacyl tRNA to the A-site of ribosomes. Induces cold shock-response and enhances P450 expression in bacteria.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Primary Target: binding of aminoacyl tRNA to the A-site of ribosomes
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      CAS #Empirical Formula
      64-75-5C₂₂H₂₄N₂O₈
      Description
      OverviewBroad-based antibiotic agent that blocks protein synthesis by inhibiting binding of aminoacyl tRNA to the A-site of ribosomes. Induces cold shock-response and enhances P450 expression in bacteria.
      Catalogue Number58346
      Brand Family Calbiochem®
      References
      ReferencesKusano, K., et al. 1999. Arch. Biochem. Biophys. 367, 129.
      Lech, K. and Brent, R. 1995. In Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al. eds.) John Wiley & Sons, New York Unit 1.4.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York, p. 1.6, A.6.
      Chopra, I. and Howe, T.G.B. 1978. Microbiol. Rev. 42, 707.
      Product Information
      CAS number64-75-5
      Activity≥900 µg/mg
      FormYellow crystalline solid
      Hill FormulaC₂₂H₂₄N₂O₈
      Chemical formulaC₂₂H₂₄N₂O₈
      Structure formula ImageStructure formula Image
      Quality LevelMQ200
      Applications
      Biological Information
      Primary Targetbinding of aminoacyl tRNA to the A-site of ribosomes
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSQI9100000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for 1 month at -20°C. Agar plates are stable for 2 weeks at 4°C. Autoclaved media should be cooled to ≤55°C before adding tetracycline.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      58346 0

      Documentation

      Tetracycline, Hydrochloride - CAS 64-75-5 - Calbiochem FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Tetracycline, Hydrochloride - CAS 64-75-5 - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      58346

      Références bibliographiques

      Aperçu de la référence bibliographique
      Kusano, K., et al. 1999. Arch. Biochem. Biophys. 367, 129.
      Lech, K. and Brent, R. 1995. In Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al. eds.) John Wiley & Sons, New York Unit 1.4.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York, p. 1.6, A.6.
      Chopra, I. and Howe, T.G.B. 1978. Microbiol. Rev. 42, 707.

      Brochure

      Titre
      Antibiotics Profiler
      Overnight Express Autoinduction Systems
      Overnight Express Autoinduction Systems

      Citations

      Titre
    • Thomas S. Lendvay, et al. (2007) Compensatory paracrine mechanisms that define the urothelial response to injury in partial bladder outlet obstruction. American Journal of Physiology: Renal 293, F1147-F1156.
      		•
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-September-2007 JSW
      DescriptionAn antibiotic that inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, preventing binding of aminoacyl tRNA to the A-site and blocking translocation. Used commonly for selection of the bacterial tetr gene whose product, a 399-amino acid membrane-associated protein, functions in active export of the drug from the cell.
      FormYellow crystalline solid
      CAS number64-75-5
      RTECSQI9100000
      Chemical formulaC₂₂H₂₄N₂O₈
      Structure formulaStructure formula
      Activity≥900 µg/mg
      SolubilityEthanol (5 mg/ml) or H₂O (20 mg/ml)
      Storage Protect from moisture
      Protect from light
      +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for 1 month at -20°C. Agar plates are stable for 2 weeks at 4°C. Autoclaved media should be cooled to ≤55°C before adding tetracycline.
      Toxicity Standard Handling
      Merck USA index14, 9196
      ReferencesKusano, K., et al. 1999. Arch. Biochem. Biophys. 367, 129.
      Lech, K. and Brent, R. 1995. In Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al. eds.) John Wiley & Sons, New York Unit 1.4.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York, p. 1.6, A.6.
      Chopra, I. and Howe, T.G.B. 1978. Microbiol. Rev. 42, 707.
      Citation
    • Thomas S. Lendvay, et al. (2007) Compensatory paracrine mechanisms that define the urothelial response to injury in partial bladder outlet obstruction. American Journal of Physiology: Renal 293, F1147-F1156.
      		•