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71540 Tag•off™ rEK Cleavage/Capture Kit

71540
  
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      Aperçu

      Replacement Information
      Description
      Overview

      This product has been discontinued.

      Tag•off™ rEK Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins, followed by rapid affinity-based capture and removal of enterokinase. Following cleavage of the target protein, greater than 99% of Tag•off rEK can be quickly removed from the reaction by affinity capture on EKapture Agarose available in the Tag•off rEK Cleavage Capture Kit. Just add EKapture Agarose to the rEK digest, incubate at room temperature for 5 minutes and remove the resin by spin filtration. Because the same buffer conditions are used for both cleavage and capture, no buffer change is necessary. The Tag•off rEK Cleavage Capture Kit includes the EKapture Agarose and Spin Filters in addition to the same components available with the Tag•off High Activity rEK (enzyme, dilution buffer, cleavage buffer and control protein).

      Catalogue Number71540
      Brand Family Novagen®
      References
      ReferencesGasparian, M.E., et al. 2003. Protein Expr. and Purif. 31, 133.
      Product Information
      50 UTag•Off™ rEK Cleavage Capture Kit
      10 µgCleavage Control Protein
      2 ml1X rEK Dilution/Storage Buffer
      5 ml10X rEK Cleavage/Capture Buffer
      1.5 mlEKapture™ Agarose
      10Spin Filter, 2 ml
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      71540 0

      Documentation

      Tag•off™ rEK Cleavage/Capture Kit Certificats d'analyse

      TitreNuméro de lot
      71540

      Références bibliographiques

      Aperçu de la référence bibliographique
      Gasparian, M.E., et al. 2003. Protein Expr. and Purif. 31, 133.

      Protocoles Utilisateur

      Titre
      TB458 Tag•off™ High Activity rEK