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561511 SENP8, His•Tag®, Human, Recombinant, E. coli

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
561511
  
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      Aperçu

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full length recombinant, human SENP8 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Structural and biological studies have shown that SENP8 is a highly specific de-NEDDylating enzyme. The protease is useful for the study of SENP8 and peptidase activity and for inhibitor screening.
      Catalogue Number561511
      Brand Family Calbiochem®
      References
      ReferencesDrag, M., et al. 2008. Biochem J. 409, 461.
      Xirodimas, D.P., et al. 2008. EMBO Rep. 9, 280.
      Mikolajczyk, J., et al. 2007. J Biol. Chem. 282, 26217.
      Mendoza, H.M., et al. 2003.J Biol Chem. 2003. 278, 25637.
      Product Information
      ActivityEC₅₀≥0.9 nM as measured in an endopeptidase assay using the substrate Nedd8 per Mikolajczyk, et al.
      FormLiquid
      FormulationIn 200 mM Imidazole, 100 mM NaCl, 50 mM HEPES, 5% Sucrose, pH 7.8.
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      561511 0

      Documentation

      SENP8, His•Tag®, Human, Recombinant, E. coli Certificats d'analyse

      TitreNuméro de lot
      561511

      Références bibliographiques

      Aperçu de la référence bibliographique
      Drag, M., et al. 2008. Biochem J. 409, 461.
      Xirodimas, D.P., et al. 2008. EMBO Rep. 9, 280.
      Mikolajczyk, J., et al. 2007. J Biol. Chem. 282, 26217.
      Mendoza, H.M., et al. 2003.J Biol Chem. 2003. 278, 25637.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-March-2009 JSW
      DescriptionFull length recombinant, human SENP8 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Structural and biological studies have shown that SENP8 is a highly specific de-NEDDylating enzyme. The protease is useful for the study of SENP8 and peptidase activity and for inhibitor screening.
      FormLiquid
      FormulationIn 200 mM Imidazole, 100 mM NaCl, 50 mM HEPES, 5% Sucrose, pH 7.8.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      ActivityEC₅₀≥0.9 nM as measured in an endopeptidase assay using the substrate Nedd8 per Mikolajczyk, et al.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesDrag, M., et al. 2008. Biochem J. 409, 461.
      Xirodimas, D.P., et al. 2008. EMBO Rep. 9, 280.
      Mikolajczyk, J., et al. 2007. J Biol. Chem. 282, 26217.
      Mendoza, H.M., et al. 2003.J Biol Chem. 2003. 278, 25637.